PSR Antibody (H-7): sc-28348

PSR Antibody (H-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects PSR protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-PSR antibody (H-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The phosphatidylserine receptor (PSR) plays a crucial role in the recognition and clearance of apoptotic cells, as PSR specifically binds to phosphatidylserine, which becomes exposed on the outer membrane of cells undergoing apoptosis. This interaction is vital for the phagocytosis of dying cells by macrophages and dendritic cells, thereby preventing inflammation and maintaining tissue homeostasis. PSR is predominantly expressed on the surface of macrophages, fibroblasts, and epithelial cells, with high expression levels noted in heart, skeletal muscle, and kidney tissues. Additionally, PSR is extensively glycosylated, which may influence binding properties and interactions with other proteins. The conservation of PSR across species, as evidenced by significant homology to proteins found in Caenorhabditis elegans and Drosophila melanogaster, underscores PSR′s fundamental role in cellular processes throughout evolution.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PSR Antibody (H-7) References:

1. The role of phosphatidylserine in recognition of apoptotic cells by phagocytes.  |  Fadok, VA., et al. 1998. Cell Death Differ. 5: 551-62. PMID: 10200509
2. Exposure of phosphatidylserine is a general feature in the phagocytosis of apoptotic lymphocytes by macrophages.  |  Krahling, S., et al. 1999. Cell Death Differ. 6: 183-9. PMID: 10200565
3. Requirement for croquemort in phagocytosis of apoptotic cells in Drosophila.  |  Franc, NC., et al. 1999. Science. 284: 1991-4. PMID: 10373118
4. Apoptosis. Gone but not forgotten.  |  Green, DR. and Beere, HM. 2000. Nature. 405: 28-9. PMID: 10811203
5. A receptor for phosphatidylserine-specific clearance of apoptotic cells.  |  Fadok, VA., et al. 2000. Nature. 405: 85-90. PMID: 10811223
6. Nuclear localization of the phosphatidylserine receptor protein via multiple nuclear localization signals.  |  Cui, P., et al. 2004. Exp Cell Res. 293: 154-63. PMID: 14729065
7. Phosphatidylserine receptor cooperates with high-density lipoprotein receptor in recognition of apoptotic cells by thymic nurse cells.  |  Cao, WM., et al. 2004. J Mol Endocrinol. 32: 497-505. PMID: 15072554
8. Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages.  |  Fadok, VA., et al. 1992. J Immunol. 148: 2207-16. PMID: 1545126
9. Interaction between phosphatidylserine and the phosphatidylserine receptor inhibits immune responses in vivo.  |  Hoffmann, PR., et al. 2005. J Immunol. 174: 1393-404. PMID: 15661897
10. Phosphatidylserine receptor Tim-4 is essential for the maintenance of the homeostatic state of resident peritoneal macrophages.  |  Wong, K., et al. 2010. Proc Natl Acad Sci U S A. 107: 8712-7. PMID: 20421466
11. p85α recruitment by the CD300f phosphatidylserine receptor mediates apoptotic cell clearance required for autoimmunity suppression.  |  Tian, L., et al. 2014. Nat Commun. 5: 3146. PMID: 24477292
12. Candidate adaptor protein CED-6 promotes the engulfment of apoptotic cells in C. elegans.  |  Liu, QA. and Hengartner, MO. 1998. Cell. 93: 961-72. PMID: 9635426

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PSR Antibody (H-7) has 32 citations in a variety of scientific publications.

Primary antibodies like Anti-PSR Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PSR Antibody (H-7) Specifications

Alternate Product Name: JMJD6

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: PSR

Epitope Species: human

Product Reactivity/Detection: PSR

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 32 citations in various scientific publications

Available Conjugates/Forms for PSR Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin