PSPC1 Antibody (G-7): sc-374387

PSPC1 Antibody (G-7) is a mouse monoclonal IgG3 kappa light chain antibody that detects PSPC1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-PSPC1 antibody (G-7) is available as the non-conjugated form. PSPC1, or paraspeckle component 1, is a crucial 523 amino acid protein that plays a significant role in cellular processes by localizing to both the cytoplasm and the nuclear matrix, where PSPC1 is involved in the formation of paraspeckles—nuclear structures that sequester specific RNA molecules and proteins. This localization is vital for the regulation of gene expression, particularly in response to stress and during cellular differentiation. PSPC1 contains two RNA recognition motif domains, which facilitate PSPC1′s interaction with RNA and other proteins, including the ability to form heterodimers with p54/nrb, thereby influencing androgen receptor-mediated gene transcription activity, especially in Sertoli cell lines. The gene encoding PSPC1 is located on human chromosome 13, a region associated with several critical genes, including BRCA2 and RB1, and is implicated in various genetic disorders, such as Trisomy 13, which can lead to severe developmental issues. The presence of alternatively spliced isoforms, alpha and beta, further adds complexity to PSPC1′s function, as these isoforms are subject to DNA damage-dependent phosphorylation, likely mediated by ATR or ATM, highlighting PSPC1′s role in cellular response to DNA damage.

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PSPC1 Antibody (G-7) References:

1. Directed proteomic analysis of the human nucleolus.  |  Andersen, JS., et al. 2002. Curr Biol. 12: 1-11. PMID: 11790298
2. Paraspeckles: a novel nuclear domain.  |  Fox, AH., et al. 2002. Curr Biol. 12: 13-25. PMID: 11790299
3. p54nrb acts as a transcriptional coactivator for activation function 1 of the human androgen receptor.  |  Ishitani, K., et al. 2003. Biochem Biophys Res Commun. 306: 660-5. PMID: 12810069
4. Expression and functional significance of mouse paraspeckle protein 1 on spermatogenesis.  |  Myojin, R., et al. 2004. Biol Reprod. 71: 926-32. PMID: 15140795
5. Nucleolar proteome dynamics.  |  Andersen, JS., et al. 2005. Nature. 433: 77-83. PMID: 15635413
6. P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner.  |  Fox, AH., et al. 2005. Mol Biol Cell. 16: 5304-15. PMID: 16148043
7. PSPC1, NONO, and SFPQ are expressed in mouse Sertoli cells and may function as coregulators of androgen receptor-mediated transcription.  |  Kuwahara, S., et al. 2006. Biol Reprod. 75: 352-9. PMID: 16641145
8. Crystallization of a paraspeckle protein PSPC1-NONO heterodimer.  |  Passon, DM., et al. 2011. Acta Crystallogr Sect F Struct Biol Cryst Commun. 67: 1231-4. PMID: 22102035
9. Depletion of Paraspeckle Protein 1 Enhances Methyl Methanesulfonate-Induced Apoptosis through Mitotic Catastrophe.  |  Gao, X., et al. 2016. PLoS One. 11: e0146952. PMID: 26785254
10. Crystal structure of a SFPQ/PSPC1 heterodimer provides insights into preferential heterodimerization of human DBHS family proteins.  |  Huang, J., et al. 2018. J Biol Chem. 293: 6593-6602. PMID: 29530979
11. Obstructive sleep apnoea is related to melanoma aggressiveness through paraspeckle protein-1 upregulation.  |  Cubillos-Zapata, C., et al. 2023. Eur Respir J. 61: PMID: 36265878
12. K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis.  |  Zhang, XL., et al. 2023. Nat Commun. 14: 3815. PMID: 37369679

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PSPC1 Antibody (G-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-PSPC1 Antibody (G-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PSPC1 Antibody (G-7) Specifications

Alternate Product Name: PSPC1

Product Clone Name: G-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 471-512

Epitope Target: PSPC1

Epitope Species: human

Product Reactivity/Detection: PSPC1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for PSPC1 Antibody (G-7): P: Blocking Peptide

The preferred PSPC1 antibody is PSPC1 Antibody (C-3): sc-374181.