PSIP1 CRISPR/Cas9 KO Plasmid (h): sc-404589
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PSIP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PSIP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PSIP1 Antibody (3F7): sc-101087
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PSIP1 CRISPR/Cas9 KO Plasmid (h) | sc-404589 | 20 µg | $397.00 |
Overview
PSIP1 (also known as LEDGF/p75) is a chromatin-associated transcriptional cofactor that binds H3K36me3-marked active genes through its PWWP domain and helps organize transcriptional elongation, RNA processing, and DNA damage responses. By interacting with factors involved in replication stress and double-strand break repair, PSIP1 contributes to genome stability and cell survival under genotoxic conditions. PSIP1 is also recognized for tethering viral integrase to chromatin, shaping integration site selection, and it participates in regulatory programs linked to oncogenic transcription and cellular stress signaling. Dysregulation of PSIP1-associated chromatin interactions has been studied in contexts such as hematologic malignancies and other cancers where transcriptional control and repair pathway balance are altered.
PSIP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PSIP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PSIP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PSIP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PSIP1 protein expression.
This CRISPR knockout system enables efficient generation of PSIP1-deficient cell models for investigation of PSIP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PSIP1 exon(s) critical for PSIP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PSIP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PSIP1 CRISPR/Cas9 KO Plasmid (h) and PSIP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PSIP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PSIP1 HDR Plasmid (h) and PSIP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PSIP1 homology arms to support homology-directed repair at defined PSIP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.