PSIP1 Antibody (3F7): sc-101087

PSIP1 Antibody (3F7) is a mouse monoclonal IgG1 kappa light chain antibody that detects PSIP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-PSIP1 antibody (3F7) is available as a non-conjugated format. PSIP1, also known as PC4 and SFRS1 interacting protein 1, CLL-associated antigen KW-7, PSIP2, lens epithelium-derived growth factor (LEDGF), PAIP, and DFS70 (dense fine speckles 70 kDa protein), is a crucial 530 amino acid nuclear protein that plays a significant role in the regulation of gene expression. PSIP1 functions primarily as a transcriptional coactivator, forming complexes with human immunodeficiency virus type 1 (HIV-1) integrase, which is essential for nuclear localization and chromosomal association of viral proteins. PSIP1 serves as the primary integrase-to-chromatin tethering factor for HIV-1, facilitating cellular trafficking of lentiviral integrases and thereby influencing viral replication. During apoptosis, PSIP1 undergoes cleavage at three sites by caspase-3 and caspase-7, which has implications in the pathogenesis of atopic disorders, highlighting PSIP1′s multifaceted role in both viral biology and cellular processes.

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PSIP1 Antibody (3F7) References:

1. Lens epithelium-derived growth factor (LEDGF/p75) and p52 are derived from a single gene by alternative splicing.  |  Singh, DP., et al. 2000. Gene. 242: 265-73. PMID: 10721720
2. Caspase cleavage of the nuclear autoantigen LEDGF/p75 abrogates its pro-survival function: implications for autoimmunity in atopic disorders.  |  Wu, X., et al. 2002. Cell Death Differ. 9: 915-25. PMID: 12181742
3. Identification of tumor-associated antigens in chronic lymphocytic leukemia by SEREX.  |  Krackhardt, AM., et al. 2002. Blood. 100: 2123-31. PMID: 12200376
4. HIV-1 integrase forms stable tetramers and associates with LEDGF/p75 protein in human cells.  |  Cherepanov, P., et al. 2003. J Biol Chem. 278: 372-81. PMID: 12407101
5. LEDGF/p75 determines cellular trafficking of diverse lentiviral but not murine oncoretroviral integrase proteins and is a component of functional lentiviral preintegration complexes.  |  Llano, M., et al. 2004. J Virol. 78: 9524-37. PMID: 15308744
6. Identification of an evolutionarily conserved domain in human lens epithelium-derived growth factor/transcriptional co-activator p75 (LEDGF/p75) that binds HIV-1 integrase.  |  Cherepanov, P., et al. 2004. J Biol Chem. 279: 48883-92. PMID: 15371438
7. Lens epithelium-derived growth factor/p75 prevents proteasomal degradation of HIV-1 integrase.  |  Llano, M., et al. 2004. J Biol Chem. 279: 55570-7. PMID: 15475359
8. Identification of the LEDGF/p75 HIV-1 integrase-interaction domain and NLS reveals NLS-independent chromatin tethering.  |  Vanegas, M., et al. 2005. J Cell Sci. 118: 1733-43. PMID: 15797927
9. Isolation of cDNAs encoding novel transcription coactivators p52 and p75 reveals an alternate regulatory mechanism of transcriptional activation.  |  Ge, H., et al. 1998. EMBO J. 17: 6723-9. PMID: 9822615

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PSIP1 Antibody (3F7) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-PSIP1 Antibody (3F7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PSIP1 Antibody (3F7) Specifications

Alternate Product Name: PSIP1

Product Clone Name: 3F7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-51

Epitope Target: PSIP1

Epitope Species: human

Product Reactivity/Detection: PSIP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for PSIP1 Antibody (3F7): N/A