Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PSF CRISPR/Cas9 KO Plasmid (m): sc-427964
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PSF CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PSF genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PSF Antibody (D-8): sc-271796
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PSF CRISPR/Cas9 KO Plasmid (m) | sc-427964 | 20 µg | $397.00 |
Overview
Sfpq encodes the splicing factor proline- and glutamine-rich protein PSF, a multifunctional RNA- and DNA-binding regulator that coordinates pre-mRNA splicing, transcriptional control, and nuclear paraspeckle biology through interactions with long noncoding RNAs such as NEAT1. In mouse cells, PSF contributes to RNA processing fidelity, alternative splicing decisions, and coupling between transcription and RNA maturation, while also participating in genome maintenance processes linked to DNA damage responses. Through these activities, Sfpq influences broad gene expression programs affecting cell cycle control, neuronal differentiation, and stress-adaptive signaling. Dysregulated SFPQ/PSF function and mislocalization have been associated with altered RNA metabolism and neurodegeneration-relevant phenotypes, making it a useful target for mechanistic studies of RNA-binding protein networks.
PSF CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sfpq gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sfpq together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sfpq open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PSF protein expression.
This CRISPR knockout system enables efficient generation of Sfpq-deficient cell models for investigation of PSF signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sfpq exon(s) critical for PSF function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sfpq genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PSF CRISPR/Cas9 KO Plasmid (m) and PSF CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sfpq locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PSF HDR Plasmid (m) and PSF HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sfpq homology arms to support homology-directed repair at defined Sfpq target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.