PSF Antibody (D-8): sc-271796

PSF Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects PSF in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PSF Antibody (D-8) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PSF plays a crucial role in pre-mRNA splicing, a vital process for post-transcriptional regulation of gene expression, where intron sequences are removed from precursor mRNA. This splicing process is facilitated by the spliceosome, a large ribonucleoprotein complex, and PSF is essential for spliceosome formation, particularly in early stages. PSF contains two RNA recognition motifs, a proline- and glutamine-rich amino-terminal domain, and two carboxy-terminal nuclear localization signals, which are critical for function and localization. PSF is predominantly found in nucleus speckles distinct from nucleoli, and localization is influenced by the second RNA recognition motif. PSF interacts with DNA binding domains of thyroid hormone receptors and retinoic acid receptors, functioning as a repressor by recruiting histone deacetylases to these domains. PSF is expressed in developing neurons, suggesting involvement in neuronal differentiation and maturation, and undergoes proteolytic cleavage in myeloid cells to produce a shorter fragment, highlighting a dynamic role in cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PSF Antibody (D-8) References:

1. Polypyrimidine tract-binding protein-associated splicing factor is a negative regulator of transcriptional activity of the porcine p450scc insulin-like growth factor response element.  |  Urban, RJ., et al. 2000. Mol Endocrinol. 14: 774-82. PMID: 10847580
2. Identification of the polypyrimidine tract binding protein-associated splicing factor.p54(nrb) complex as a candidate DNA double-strand break rejoining factor.  |  Bladen, CL., et al. 2005. J Biol Chem. 280: 5205-10. PMID: 15590677
3. Transcriptional activity of the murine retinol-binding protein gene is regulated by a multiprotein complex containing HMGA1, p54 nrb/NonO, protein-associated splicing factor (PSF) and steroidogenic factor 1 (SF1)/liver receptor homologue 1 (LRH-1).  |  Bianconcini, A., et al. 2009. Int J Biochem Cell Biol. 41: 2189-203. PMID: 19389484
4. The splicing factor proline-glutamine rich (SFPQ/PSF) is involved in influenza virus transcription.  |  Landeras-Bueno, S., et al. 2011. PLoS Pathog. 7: e1002397. PMID: 22114566
5. Arginine methylation and citrullination of splicing factor proline- and glutamine-rich (SFPQ/PSF) regulates its association with mRNA.  |  Snijders, AP., et al. 2015. RNA. 21: 347-59. PMID: 25605962
6. Dysregulation of spliceosome gene expression in advanced prostate cancer by RNA-binding protein PSF.  |  Takayama, KI., et al. 2017. Proc Natl Acad Sci U S A. 114: 10461-10466. PMID: 28893982
7. Amyloid precursor protein, an androgen-regulated gene, is targeted by RNA-binding protein PSF/SFPQ in neuronal cells.  |  Takayama, KI., et al. 2019. Genes Cells. 24: 719-730. PMID: 31541592
8. Insight into the Tumor Suppression Mechanism from the Structure of Human Polypyrimidine Splicing Factor (PSF/SFPQ) Complexed with a 30mer RNA from Murine Virus-like 30S Transcript-1.  |  Wang, J., et al. 2022. Biochemistry. 61: 1723-1734. PMID: 35998361
9. Identification of small-molecule inhibitors against the interaction of RNA-binding protein PSF and its target RNA for cancer treatment.  |  Takayama, KI., et al. 2023. PNAS Nexus. 2: pgad203. PMID: 37388923
10. PSF-lncRNA interaction as a target for novel targeted anticancer therapies.  |  Liu, R., et al. 2024. Biomed Pharmacother. 180: 117491. PMID: 39332189

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PSF Antibody (D-8) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-PSF Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PSF Antibody (D-8) Specifications

Alternate Product Name: PSF

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 462-479

Epitope Target: PSF

Epitope Species: human

Product Reactivity/Detection: PSF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, bovine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for PSF Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred PSF antibody is PSF Antibody (39-1): sc-101137.