PRX V Antibody (B-7) recommended for detection of PRX V of human origin by WB, IP, IF, IHC(P) and ELISA

PRX V Antibody (B-7): sc-133073

PRX V Antibody (B-7) is rated 5.0 out of 5 by 7.
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Datasheets

    • PRX V Antibody (B-7) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-214 representing full length PRX V of human origin
    • recommended for detection of PRX V of human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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I am using PRX V (B-7): sc-133073 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from satisfied bands PRX V antibody works well for WB at the concentration of 1:800, with satisfied bands.
Date published: 2017-05-28
Rated 5 out of 5 by from clear band. Western blotting detection of good human cells, WB diluted 1:1000 clear band.
Date published: 2017-05-28
Rated 5 out of 5 by from worked really well This antibody worked really well for us in both WB and IF. Very pleased result, strong signal for both.
Date published: 2017-02-26
Rated 5 out of 5 by from publish using Used in WB, got correct MW band at 1:1000 dilution in HeLa whole cell lysates.
Date published: 2017-02-11
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human bronchus tissue. -SCBT QC
Date published: 2015-03-15
Rated 5 out of 5 by from Great specificity in WB in HeLa Great specificity in WB in HeLa, SHP-77 and Hep G2 whole cell lysates. -SCBT QC
Date published: 2015-02-24
Rated 5 out of 5 by from Good clear band seen in WB in Hep G2 whole Good clear band seen in WB in Hep G2 whole cell lysate. -SCBT QC
Date published: 2015-01-21
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