Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PRX III CRISPR/Cas9 KO Plasmid (h): sc-401730
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRX III CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PRX III genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PRX III Antibody (1): sc-130336
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRX III CRISPR/Cas9 KO Plasmid (h) | sc-401730 | 20 µg | $397.00 |
Overview
PRDX3 encodes peroxiredoxin III (PRX III), a mitochondria-localized thioredoxin-dependent peroxidase that detoxifies hydrogen peroxide and lipid hydroperoxides to preserve redox balance. By buffering mitochondrial reactive oxygen species, PRX III supports oxidative phosphorylation, limits oxidative damage to mitochondrial DNA and proteins, and influences redox-sensitive signaling and apoptosis. PRDX3 activity intersects with mitochondrial antioxidant systems such as thioredoxin 2/thioredoxin reductase and contributes to maintenance of mitochondrial integrity under metabolic and inflammatory stress. Altered PRDX3 expression or function has been associated with oxidative stress phenotypes and is studied in the context of disorders where mitochondrial redox dysregulation, cell survival signaling, and metabolic reprogramming are implicated.
PRX III CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PRDX3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PRDX3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PRDX3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PRX III protein expression.
This CRISPR knockout system enables efficient generation of PRDX3-deficient cell models for investigation of PRX III signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PRDX3 exon(s) critical for PRX III function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PRDX3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PRX III CRISPR/Cas9 KO Plasmid (h) and PRX III CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PRDX3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PRX III HDR Plasmid (h) and PRX III HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PRDX3 homology arms to support homology-directed repair at defined PRDX3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.