PRP6 Antibody (D-3): sc-271866
- PRP6 Antibody (D-3) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 19-47 near the N-terminus of PRP6 of human origin
- recommended for detection of PRP6 of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine, porcine and avian
- TransCruz reagent for ChIP application (sc-271866 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PRP6 Antibody (D-3) for WB applications. These reagents are now offered in bundles with PRP6 Antibody (D-3) (see ordering information below).
PRP6 Antibody (D-3) is a mouse monoclonal IgG1 antibody that detects PRP6 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PRP6 plays a crucial role in the assembly of pre-mRNA spliceosomes, specifically by facilitating the interaction between small nuclear ribonucleoproteins (snRNPs) U4/U6 and U5 to form the essential [U4/U6.U5] tri-snRNP complex. This interaction is vital for the splicing process, which is necessary for the maturation of pre-mRNA into functional mRNA, thereby influencing gene expression and protein synthesis. PRP6 is predominantly localized in discrete subnuclear regions, mirroring the localization patterns observed in mammalian splicing components, which underscores PRP6′s importance in the regulation of splicing dynamics. Notably, mammalian PRP6, isolated from HeLa nuclear extracts, shares conserved tetrarico peptide repeats with its yeast counterpart, Prp6p, establishing PRP6 as the mammalian homolog. Unlike yeast Prp6p, which is specific to U4/U6, human PRP6 engages in protein-protein interactions within the tri-snRNP, connecting both U5 and U4/U6 snRNPs, thus playing a pivotal role in the splicing machinery. Anti-PRP6 antibody (D-3) is an invaluable tool for researchers studying RNA splicing and its regulatory mechanisms in various biological contexts.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRP6 Antibody (D-3) | sc-271866 | 200 µg/ml | $322.00 | |||
PRP6 Antibody (D-3): m-IgG Fc BP-HRP Bundle | sc-540269 | 200 µg Ab; 10 µg BP | $361.00 | |||
PRP6 Antibody (D-3): m-IgG1 BP-HRP Bundle | sc-542014 | 200 µg Ab; 20 µg BP | $361.00 | |||
PRP6 (D-3) Neutralizing Peptide | sc-271866 P | 100 µg/0.5 ml | $69.00 | |||
PRP6 Antibody (D-3) X | sc-271866 X | 200 µg/0.1 ml | $322.00 |