PRP6 Antibody (B-1): sc-166889

PRP6 Antibody (B-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects PRP6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PRP6 (B-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PRP6 plays a crucial role in the assembly of pre-mRNA spliceosomes, which are essential for the splicing of precursor mRNA into mature mRNA, a process vital for gene expression regulation. Specifically, PRP6 is involved in the formation of the [U4/U6.U5] tri-snRNP complex, which is necessary for the splicing process. In yeast, the homologous protein Prp6p is required for the accumulation of this tri-snRNP, and Prp6p is localized in discrete subnuclear regions, mirroring the localization of mammalian splicing components. Mammalian PRP6, isolated from HeLa nuclear extracts, shares conserved tetratricopeptide repeats with yeast Prp6p, establishing PRP6 as the mammalian counterpart. Unlike yeast Prp6p, human PRP6 interacts with both U5 and U4/U6 snRNPs, facilitating critical protein-protein interactions that bridge these two snRNP particles, thereby enhancing the efficiency of spliceosome assembly and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PRP6 Antibody (B-1) References:

1. The human homologue of the yeast splicing factor prp6p contains multiple TPR elements and is stably associated with the U5 snRNP via protein-protein interactions.  |  Makarov, EM., et al. 2000. J Mol Biol. 298: 567-75. PMID: 10788320
2. A yeast splicing factor is localized in discrete subnuclear domains.  |  Elliott, DJ., et al. 1992. EMBO J. 11: 3731-6. PMID: 1396568
3. PRP38 encodes a yeast protein required for pre-mRNA splicing and maintenance of stable U6 small nuclear RNA levels.  |  Blanton, S., et al. 1992. Mol Cell Biol. 12: 3939-47. PMID: 1508195
4. The yeast PRP6 gene encodes a U4/U6 small nuclear ribonucleoprotein particle (snRNP) protein, and the PRP9 gene encodes a protein required for U2 snRNP binding.  |  Abovich, N., et al. 1990. Mol Cell Biol. 10: 6417-25. PMID: 2147224
5. The biochemical defects of prp4-1 and prp6-1 yeast splicing mutants reveal that the PRP6 protein is required for the accumulation of the [U4/U6.U5] tri-snRNP.  |  Galisson, F. and Legrain, P. 1993. Nucleic Acids Res. 21: 1555-62. PMID: 8479905

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PRP6 Antibody (B-1) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-PRP6 Antibody (B-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PRP6 Antibody (B-1) Specifications

Alternate Product Name: PRP6

Product Clone Name: B-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: PRP6

Epitope Species: human

Product Reactivity/Detection: PRP6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for PRP6 Antibody (B-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent