PRP19 Antibody (G-7): sc-514338
- PRP19 Antibody (G-7) is a mouse monoclonal IgG2a κ PRP19 antibody, cited in 9 publications, provided at 200 µg/ml
- raised against amino acids 141-300 mapping within an internal region of PRP19 of human origin
- PRP19 Antibody (G-7) is recommended for detection of PRP19 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PRP19 Antibody (G-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PRP19 Antibody (G-7) for WB applications. These reagents are now offered in bundles with PRP19 Antibody (G-7) (see ordering information below).
PRP19 Antibody (G-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects PRP19 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PRP19 Antibody (G-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PRP19 protein plays a crucial role in the spliceosome, a large and complex molecular machine responsible for pre-mRNA splicing in eukaryotic cells. This process is vital for the removal of non-coding regions, known as introns, from pre-mRNA, allowing the remaining coding sequences, or exons, to be joined together to form mature mRNA, which is then translated into proteins. PRP19-associated complex is essential for the stable association of U5 and U6 snRNPs with the spliceosome following the release of U4, facilitating critical structural changes that enhance the interaction between U6 and the intron sequence. By understanding the function of PRP19 and its interactions within the spliceosome, researchers can gain insights into the regulation of gene expression and the potential implications of splicing errors in various diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PRP19 Antibody (G-7) References:
- The spliceosome: the most complex macromolecular machine in the cell? | Nilsen, TW. 2003. Bioessays. 25: 1147-9. PMID: 14635248
- Roles of the U5 snRNP in spliceosome dynamics and catalysis. | Turner, IA., et al. 2004. Biochem Soc Trans. 32: 928-31. PMID: 15506927
- Towards understanding the catalytic core structure of the spliceosome. | Butcher, SE. and Brow, DA. 2005. Biochem Soc Trans. 33: 447-9. PMID: 15916538
- The Prp19-associated complex is required for specifying interactions of U5 and U6 with pre-mRNA during spliceosome activation. | Chan, SP. and Cheng, SC. 2005. J Biol Chem. 280: 31190-9. PMID: 15994330
- snRNAs as the catalysts of pre-mRNA splicing. | Valadkhan, S. 2005. Curr Opin Chem Biol. 9: 603-8. PMID: 16242989
- Functional links between the Prp19-associated complex, U4/U6 biogenesis, and spliceosome recycling. | Chen, CH., et al. 2006. RNA. 12: 765-74. PMID: 16540691
- Functional association of essential splicing factor(s) with PRP19 in a protein complex. | Tarn, WY., et al. 1994. EMBO J. 13: 2421-31. PMID: 8194532
- Yeast precursor mRNA processing protein PRP19 associates with the spliceosome concomitant with or just after dissociation of U4 small nuclear RNA. | Tarn, WY., et al. 1993. Proc Natl Acad Sci U S A. 90: 10821-5. PMID: 8248176
- PRP19: a novel spliceosomal component. | Cheng, SC., et al. 1993. Mol Cell Biol. 13: 1876-82. PMID: 8441419
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRP19 Antibody (G-7) | sc-514338 | 200 µg/ml | $322.00 | |||
PRP19 Antibody (G-7): m-IgG Fc BP-HRP Bundle | sc-531094 | 200 µg Ab; 10 µg BP | $361.00 | |||
PRP19 Antibody (G-7): m-IgGκ BP-HRP Bundle | sc-524690 | 200 µg Ab, 40 µg BP | $361.00 | |||
PRP19 Antibody (G-7): m-IgG2a BP-HRP Bundle | sc-547684 | 200 µg Ab; 10 µg BP | $361.00 | |||
PRP19 Antibody (G-7) AC | sc-514338 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PRP19 Antibody (G-7) HRP | sc-514338 HRP | 200 µg/ml | $322.00 | |||
PRP19 Antibody (G-7) FITC | sc-514338 FITC | 200 µg/ml | $336.00 | |||
PRP19 Antibody (G-7) PE | sc-514338 PE | 200 µg/ml | $349.00 | |||
PRP19 Antibody (G-7) Alexa Fluor® 488 | sc-514338 AF488 | 200 µg/ml | $364.00 | |||
PRP19 Antibody (G-7) Alexa Fluor® 546 | sc-514338 AF546 | 200 µg/ml | $364.00 | |||
PRP19 Antibody (G-7) Alexa Fluor® 594 | sc-514338 AF594 | 200 µg/ml | $364.00 | |||
PRP19 Antibody (G-7) Alexa Fluor® 647 | sc-514338 AF647 | 200 µg/ml | $364.00 | |||
PRP19 Antibody (G-7) Alexa Fluor® 680 | sc-514338 AF680 | 200 µg/ml | $364.00 | |||
PRP19 Antibody (G-7) Alexa Fluor® 790 | sc-514338 AF790 | 200 µg/ml | $364.00 |