Protein C CRISPR/Cas9 KO Plasmid (h): sc-405598
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Protein C CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Protein C genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Protein C Antibody (C-10): sc-377175
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Protein C CRISPR/Cas9 KO Plasmid (h) | sc-405598 | 20 µg | $397.00 |
Overview
PROC encodes protein C, a vitamin K–dependent serine protease zymogen synthesized in the liver that circulates as a key regulator of hemostasis. Upon activation on the endothelial surface by the thrombin–thrombomodulin complex, activated protein C proteolytically inactivates coagulation cofactors Va and VIIIa, thereby limiting thrombin generation. This anticoagulant pathway is coordinated with endothelial protein C receptor (EPCR) and protein S, linking coagulation control to vascular inflammation and barrier function. Altered PROC expression or function is associated with dysregulated coagulation homeostasis and thrombotic disease phenotypes, supporting its use in mechanistic studies of coagulation signaling and endothelial biology.
Protein C CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PROC gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PROC together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PROC open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Protein C protein expression.
This CRISPR knockout system enables efficient generation of PROC-deficient cell models for investigation of Protein C signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PROC exon(s) critical for Protein C function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PROC genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Protein C CRISPR/Cas9 KO Plasmid (h) and Protein C CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PROC locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Protein C HDR Plasmid (h) and Protein C HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PROC homology arms to support homology-directed repair at defined PROC target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.