Proteassemblin Antibody (B-1): sc-393267

Proteassemblin Antibody (B-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects Proteassemblin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Proteassemblin (B-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Proteassemblin, also known as POMP (proteasome maturation protein), UMP1, or Voltage-gated potassium channel β subunit 4.1, is primarily located in the endoplasmic reticulum (ER), where anti-Proteassemblin antibody (B-1) plays a crucial role as a molecular chaperone essential for proteasome and immunoproteasome assembly. This localization is vital because the ER is the site of protein synthesis and folding, and Proteassemblin′s function in facilitating proper proteasome assembly is critical for maintaining cellular homeostasis and regulating protein degradation. By ensuring correct incorporation of β subunits into the 20S proteasome, Proteassemblin helps prevent misfolded protein accumulation, thereby supporting cell viability and responding to stress signals such as interferon-gamma. Notably, Proteassemblin is the human homolog of yeast Ump1 protein, but unlike Ump1, which becomes part of the proteasome structure, Proteassemblin degrades once the proteasome matures, highlighting a transient yet essential role in proteasome biogenesis.

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Proteassemblin Antibody (B-1) References:

1. Identification of proteassemblin, a mammalian homologue of the yeast protein, Ump1p, that is required for normal proteasome assembly.  |  Griffin, TA., et al. 2000. Mol Cell Biol Res Commun. 3: 212-7. PMID: 10891394
2. Inhibition of proteasome activity induces concerted expression of proteasome genes and de novo formation of Mammalian proteasomes.  |  Meiners, S., et al. 2003. J Biol Chem. 278: 21517-25. PMID: 12676932
3. Protein-protein interactions among human 20S proteasome subunits and proteassemblin.  |  Jayarapu, K. and Griffin, TA. 2004. Biochem Biophys Res Commun. 314: 523-8. PMID: 14733938
4. RNA interference toward UMP1 induces proteasome inhibition in Saccharomyces cerevisiae: evidence for protein oxidation and autophagic cell death.  |  Chen, Q., et al. 2005. Free Radic Biol Med. 38: 226-34. PMID: 15607905
5. IFN-gamma-induced immune adaptation of the proteasome system is an accelerated and transient response.  |  Heink, S., et al. 2005. Proc Natl Acad Sci U S A. 102: 9241-6. PMID: 15944226
6. Analysis of the spontaneous mutator phenotype associated with 20S proteasome deficiency in S. cerevisiae.  |  McIntyre, J., et al. 2006. Mutat Res. 593: 153-63. PMID: 16095633
7. A heterodimeric complex that promotes the assembly of mammalian 20S proteasomes.  |  Hirano, Y., et al. 2005. Nature. 437: 1381-5. PMID: 16251969
8. Possible tetramerisation of the proteasome maturation factor POMP/proteassemblin/hUmp1 and its subcellular localisation.  |  Hoefer, MM., et al. 2006. Int J Biol Macromol. 38: 259-67. PMID: 16624403
9. The proteasome maturation protein POMP facilitates major steps of 20S proteasome formation at the endoplasmic reticulum.  |  Fricke, B., et al. 2007. EMBO Rep. 8: 1170-5. PMID: 17948026

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Proteassemblin Antibody (B-1) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Proteassemblin Antibody (B-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Proteassemblin Antibody (B-1) Specifications

Alternate Product Name: Proteassemblin

Product Clone Name: B-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 38-63

Epitope Target: Proteassemblin

Epitope Species: human

Product Reactivity/Detection: Proteassemblin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Proteassemblin Antibody (B-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin