ProRS Antibody (A-2): sc-393505

ProRS Antibody (A-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects ProRS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ProRS (A-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Prolyl-tRNA synthetase (ProRS) plays a crucial role in protein synthesis by ensuring accurate charging of tRNA with correct amino acids, which is vital for maintaining translation fidelity. ProRS is located in the cytoplasm, where ProRS catalyzes the attachment of proline to corresponding tRNA, thus facilitating proline incorporation into nascent polypeptide chains during protein synthesis. Proper localization of ProRS is essential, as proper localization ensures efficient and accurate aminoacylation, preventing errors that could lead to dysfunctional proteins. ProRS, also known as EPRS, EARS, PARS, QARS, QPRS, PIG32, or GLUPRORS, is a 1,512 amino acid protein characterized by three WHEP-TRS domains and belongs to both class-I and class-II aminoacyl-tRNA synthetase family. Through ATP utilization, ProRS catalyzes the conversion of L-glutamate and tRNA(Glu) to L-glutamyl-tRNA(Glu), as well as the conversion of L-proline and tRNA(Pro) to L-prolyl-tRNA(Pro), thereby playing a pivotal role in protein synthesis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ProRS Antibody (A-2) References:

1. Heat shock protein 90 mediates protein-protein interactions between human aminoacyl-tRNA synthetases.  |  Kang, J., et al. 2000. J Biol Chem. 275: 31682-8. PMID: 10913161
2. Structural analysis of multifunctional peptide motifs in human bifunctional tRNA synthetase: identification of RNA-binding residues and functional implications for tandem repeats.  |  Jeong, EJ., et al. 2000. Biochemistry. 39: 15775-82. PMID: 11123902
3. Interaction network of human aminoacyl-tRNA synthetases and subunits of elongation factor 1 complex.  |  Sang Lee, J., et al. 2002. Biochem Biophys Res Commun. 291: 158-64. PMID: 11829477
4. Noncanonical function of glutamyl-prolyl-tRNA synthetase: gene-specific silencing of translation.  |  Sampath, P., et al. 2004. Cell. 119: 195-208. PMID: 15479637
5. WHEP domains direct noncanonical function of glutamyl-Prolyl tRNA synthetase in translational control of gene expression.  |  Jia, J., et al. 2008. Mol Cell. 29: 679-90. PMID: 18374644
6. The human QARS locus: assignment of the human gene for glutaminyl-tRNA synthetase to chromosome 1q32-42.  |  Kunze, N., et al. 1990. Hum Genet. 85: 527-30. PMID: 2227938
7. The human EPRS locus (formerly the QARS locus): a gene encoding a class I and a class II aminoacyl-tRNA synthetase.  |  Kaiser, E., et al. 1994. Genomics. 19: 280-90. PMID: 8188258
8. A multifunctional repeated motif is present in human bifunctional tRNA synthetase.  |  Rho, SB., et al. 1998. J Biol Chem. 273: 11267-73. PMID: 9556618
9. Macromolecular assemblage of aminoacyl-tRNA synthetases: identification of protein-protein interactions and characterization of a core protein.  |  Quevillon, S., et al. 1999. J Mol Biol. 285: 183-95. PMID: 9878398

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ProRS Antibody (A-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-ProRS Antibody (A-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ProRS Antibody (A-2) Specifications

Alternate Product Name: ProRS

Product Clone Name: A-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1213-1512

Epitope Target: ProRS

Epitope Species: human

Product Reactivity/Detection: ProRS

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for ProRS Antibody (A-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin