Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Progesterone Receptor CRISPR/Cas9 KO Plasmid (m): sc-422214
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Progesterone Receptor CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Progesterone Receptor genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Progesterone Receptor Antibody (A-2): sc-398898
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Progesterone Receptor CRISPR/Cas9 KO Plasmid (m) | sc-422214 | 20 µg | $397.00 |
Overview
Pgr encodes the mouse progesterone receptor, a ligand-activated nuclear hormone receptor that functions as a transcription factor controlling gene programs downstream of progesterone. Upon hormone binding, PGR forms dimers, translocates to the nucleus, and regulates chromatin accessibility and RNA polymerase II recruitment to coordinate epithelial differentiation, decidualization, implantation, and mammary gland development. PGR signaling interfaces with estrogen receptor pathways, MAPK/PI3K cascades, and coregulator complexes to fine-tune cell-cycle progression, immune modulation, and tissue remodeling. Dysregulated Pgr activity or altered receptor isoform balance is widely used to model reproductive endocrine phenotypes and hormone-dependent changes relevant to uterine biology and mammary physiology.
Progesterone Receptor CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pgr gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pgr together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pgr open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Progesterone Receptor protein expression.
This CRISPR knockout system enables efficient generation of Pgr-deficient cell models for investigation of Progesterone Receptor signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pgr exon(s) critical for Progesterone Receptor function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pgr genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Progesterone Receptor CRISPR/Cas9 KO Plasmid (m) and Progesterone Receptor CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pgr locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Progesterone Receptor HDR Plasmid (m) and Progesterone Receptor HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pgr homology arms to support homology-directed repair at defined Pgr target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.