pro BDNF Antibody (5H8): sc-65514

pro BDNF Antibody (5H8) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect the precursor form of Brain-Derived Neurotrophic Factor (proBDNF), also known as preproBDNF. anti-proBDNF antibody (5H8) targets a precise epitope within amino acids 104-112 (PPLLFLEEv) of human proBDNF, ensuring accurate recognition across multiple species including mouse, rat, human, bovine, porcine, and canine. proBDNF is primarily localized within the secretory pathway of neurons, such as the endoplasmic reticulum and Golgi apparatus, where processing to mature BDNF occurs, a crucial neurotrophin involved in neuronal survival, differentiation, and synaptic plasticity. Understanding the cellular location of proBDNF is essential for elucidating its role in neural development and implications in various neurological disorders. proBDNF monoclonal antibody (5H8) performs effectively in western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) assays, making this tool indispensable for researchers studying neurotrophin signaling pathways. Available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® dyes, anti-proBDNF antibody (5H8) accommodates a wide range of experimental protocols. By providing detailed insights into cellular localization and processing of proBDNF, proBDNF (5H8) antibody supports critical research aimed at unraveling complex mechanisms governing neuronal function and brain health.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

pro BDNF Antibody (5H8) References:

1. Biosynthesis and post-translational processing of the precursor to brain-derived neurotrophic factor.  |  Mowla, SJ., et al. 2001. J Biol Chem. 276: 12660-6. PMID: 11152678
2. Neurotrophin receptors: a window into neuronal differentiation.  |  Chao, MV. 1992. Neuron. 9: 583-93. PMID: 1327010
3. Rapid BDNF-induced retrograde synaptic modification in a developing retinotectal system.  |  Du, JL. and Poo, MM. 2004. Nature. 429: 878-83. PMID: 15215865
4. BDNF from microglia causes the shift in neuronal anion gradient underlying neuropathic pain.  |  Coull, JA., et al. 2005. Nature. 438: 1017-21. PMID: 16355225
5. The changing scene of neurotrophic factors.  |  Thoenen, H. 1991. Trends Neurosci. 14: 165-70. PMID: 1713715
6. Cell biology of BDNF and its relevance to schizophrenia.  |  Lu, B. and Martinowich, K. 2008. Novartis Found Symp. 289: 119-29; discussion 129-35, 193-5. PMID: 18497099
7. Cell death during development of the nervous system.  |  Oppenheim, RW. 1991. Annu Rev Neurosci. 14: 453-501. PMID: 2031577
8. Acute exercise modulates BDNF and pro-BDNF protein content in immune cells.  |  Brunelli, A., et al. 2012. Med Sci Sports Exerc. 44: 1871-80. PMID: 22543740
9. BDNF Expression in Cortical GABAergic Interneurons.  |  Barreda Tomás, FJ., et al. 2020. Int J Mol Sci. 21: PMID: 32106593
10. Similarities and differences in the way neurotrophins interact with the Trk receptors in neuronal and nonneuronal cells.  |  Ip, NY., et al. 1993. Neuron. 10: 137-49. PMID: 7679912
11. Role of neurotrophins in mouse neuronal development.  |  Klein, R. 1994. FASEB J. 8: 738-44. PMID: 8050673
12. The neurotrophic factor concept: a reexamination.  |  Korsching, S. 1993. J Neurosci. 13: 2739-48. PMID: 8331370

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-pro BDNF Antibody (5H8) has 82 citations in a variety of scientific publications.

Primary antibodies like Anti-pro BDNF Antibody (5H8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

pro BDNF Antibody (5H8) Specifications

Alternate Product Name: BDNF

Product Clone Name: 5H8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 104-112 PPLLFLEEv

Epitope Target: pro BDNF

Epitope Species: human

Product Reactivity/Detection: BDNF preproprotein

Species Reactivity/Detection: mouse, rat, human, bovine, porcine, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 82 citations in various scientific publications

Available Conjugates/Forms for pro BDNF Antibody (5H8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred pro BDNF antibody is pro BDNF Antibody (9C1): sc-65513.