Date published: 2026-4-28

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pro BDNF Antibody (9C1): sc-65513

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Datasheets
  • pro BDNF Antibody (9C1) is a mouse monoclonal IgG1 κ, cited in 26 publications, provided at 200 µg/ml
  • raised against recombinant pro BDNF of human origin
  • Anti-pro BDNF Antibody (9C1) is recommended for detection of BDNF preproprotein of mouse, rat, human, bovine, porcine and canine origin by WB, IP and IF
  • Anti-pro BDNF Antibody (9C1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for pro BDNF Antibody (9C1) for WB applications. These reagents are now offered in bundles with pro BDNF Antibody (9C1) (see ordering information below).
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pro BDNF Antibody (9C1) is a mouse monoclonal IgG1 kappa light chain antibody engineered to detect the pro form of Brain-Derived Neurotrophic Factor (pro BDNF), also known as proBDNF or BDNF preproprotein. anti-pro BDNF antibody (9C1) is raised against recombinant human pro BDNF and exhibits broad species reactivity, effectively recognizing BDNF preproprotein from mouse, rat, human, bovine, porcine, and canine origins in applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). BDNF plays a pivotal role in the regulation of neuronal survival, differentiation, and synaptic plasticity, making BDNF essential for brain development and cognitive function. pro BDNF (9C1) monoclonal antibody allows researchers to investigate the maturation process of BDNF and its involvement in various neurodegenerative diseases and neural development pathways. anti-pro BDNF antibody (9C1) is available in both non-conjugated form and multiple conjugated variants, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and several Alexa Fluor® conjugates, providing versatile options for diverse experimental needs. Understanding BDNF′s interaction with receptors such as TrkB underscores the significance of pro BDNF monoclonal antibody (9C1) in exploring therapeutic targets for neurological disorders. pro BDNF (9C1) antibody serves as an essential reagent for advancing neuroscience research and unraveling the complexities of neurotrophic factor biology.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

pro BDNF Antibody (9C1)

sc-65513
200 µg/ml
$322.00

pro BDNF Antibody (9C1): m-IgG Fc BP-HRP Bundle

sc-526957
200 µg Ab; 10 µg BP
$361.00

pro BDNF Antibody (9C1): m-IgG1 BP-HRP Bundle

sc-532330
200 µg Ab; 20 µg BP
$361.00

pro BDNF Antibody (9C1) AC

sc-65513 AC
500 µg/ml, 25% agarose
$424.00

pro BDNF Antibody (9C1) HRP

sc-65513 HRP
200 µg/ml
$322.00

pro BDNF Antibody (9C1) FITC

sc-65513 FITC
200 µg/ml
$336.00

pro BDNF Antibody (9C1) PE

sc-65513 PE
200 µg/ml
$349.00

pro BDNF Antibody (9C1) Alexa Fluor® 488

sc-65513 AF488
200 µg/ml
$364.00

pro BDNF Antibody (9C1) Alexa Fluor® 546

sc-65513 AF546
200 µg/ml
$364.00

pro BDNF Antibody (9C1) Alexa Fluor® 594

sc-65513 AF594
200 µg/ml
$364.00

pro BDNF Antibody (9C1) Alexa Fluor® 647

sc-65513 AF647
200 µg/ml
$364.00

pro BDNF Antibody (9C1) Alexa Fluor® 680

sc-65513 AF680
200 µg/ml
$364.00

pro BDNF Antibody (9C1) Alexa Fluor® 790

sc-65513 AF790
200 µg/ml
$364.00

Is this antibody (SC 65513) detecting both pro and mature BDNF or specific for Proform only?

Asked by: Yoon
Thank you for your question. sc-65513 pro BDNF (9C1) can detect the pro and mature form .
Answered by: BlakeJ
Date published: 2026-03-19

Does Anti-pro BDNF Antibody (9C1): sc-65513 identifies pro-BDNF as well as mature BDNF? If not, do you have another antibody for the mature form?

Asked by: normandox
Thank you for your question. Anti-pro BDNF Antibody (9C1): sc-65513 recognizes a sequence within the propeptide sequence, which is not present in mature BDNF protein. As such, the antibody will not detect mature BDNF. We currently only offer BDNF precursor protein-specific antibodies.
Answered by: Tech Support Europe
Date published: 2025-04-09

Bonjour, Je vais utiliser cet anticorps (sc-65513) pour mon Western blot j'aimerais savoir dans quoi je dois le diluer SVP (BSA ou Lait)

Asked by: rahma
Merci pour votre requête. En Western Blot, nous recommandons de diluer cet anticorps dans une solution de TBS avec 5% de lait et 0,05% de Tween-20. Vous trouverez des informations complémentaires dans notre page protocoles en ligne. Thank you for your inquiry. In Western Blot, we suggest to dilute this antibody in TBS with 5% milk and 0,05% Tween-20. You will find additional information in our protocols available online.
Answered by: Technical Support Europe
Date published: 2017-02-23

Is there a blocking peptide available for this antibody?

Asked by: AbPolly
Thank you for your question. This antibody is raised against a recombinant protein and not a short peptide sequence, so it is not possible to design a blocking peptide for it.
Answered by: Technical Service 11
Date published: 2017-02-09
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Rated 1 out of 5 by from Not workingI used it with different protocols It does not work
Date published: 2019-04-29
Rated 3 out of 5 by from Funzionamento medioL’anticorpo è stato testato mediante immunoistochimica ed immunofluorescenza (1:250) su campioni di encefalo di bovino fissati in formalina ed inclusi in paraffina. Le prove hanno entrambe dato esito positivo con segnale specifico intraneuronale a livello del giro dentato dell'ippocampo e in alcuni nuclei talamici. Lo stesso anticorpo è stato testato anche mediante western blot (1:500) su omogenati di encefalo di bovino. Le prove hanno mostrato segnale aspecifico con bandeggio sopra i 50 KDa in tutte le aree cerebrali analizzate (ippocampo, corteccia frontale, c. temporale e cervelletto) e nessun segnale sotto i 50 KDa.​
Date published: 2018-12-21
Rated 5 out of 5 by from properties goodWe have also identified three additional structural homologous neurotrophic factors, replacing the traditional secondary anti-mouse IgG reagent according to the suggested recombinant for the product description. The properties are generally good
Date published: 2017-02-09
Rated 5 out of 5 by from Wonderful detectionIt will provide your desired detection in immunoblotting and staining procedures. It works with mouse, rat, human, and has been published with more than ten times.
Date published: 2017-01-27
Rated 5 out of 5 by from worthGood seller, baby is very worth buying very recommended
Date published: 2016-12-17
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pro BDNF Antibody (9C1) is rated 3.8 out of 5 by 6.
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