PRMT5 Antibody (PRMT5-21): sc-59650
- PRMT5 Antibody (PRMT5-21) is a mouse monoclonal IgG2a, cited in 2 publications, provided at 100 µg/ml
- raised against the C-terminus of PRMT5 of mouse origin
- recommended for detection of PRMT5 of mouse, rat, human, bovine and canine origin by WB
- See PRMT5 (A-11): sc-376937 for PRMT5 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PRMT5 Antibody (PRMT5-21). This work is in progress.
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PRMT5 Antibody (PRMT5-21) is a mouse monoclonal IgG2a antibody that detects PRMT5 in mouse, rat, human, bovine, and canine samples through western blotting (WB). PRMT5 functions as a type II protein-arginine methyltransferase, playing a vital role in post-translational modification of proteins through symmetrical dimethylation of arginine residues. This modification is essential for spliceosome assembly, a complex responsible for processing pre-mRNA into mature mRNA, which is crucial for gene expression regulation. PRMT5 influences transcriptional control and cellular proliferation by interacting with specific promoter regions, such as cyclin E1. PRMT5 is predominantly localized in the cytoplasm across various tissues, highlighting its significance in cellular processes. The gene encoding human PRMT5 is located on chromosome 14q11, underscoring evolutionary conservation and importance across species. With an ability to facilitate critical cellular functions, PRMT5 is a key player in maintaining normal cellular homeostasis and is implicated in various diseases when dysregulated.
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PRMT5 Antibody (PRMT5-21) References:
- The PRMT5 arginine methyltransferase: many roles in development, cancer and beyond. | Stopa, N., et al. 2015. Cell Mol Life Sci. 72: 2041-59. PMID: 25662273
- MTAP deletion confers enhanced dependency on the PRMT5 arginine methyltransferase in cancer cells. | Kryukov, GV., et al. 2016. Science. 351: 1214-8. PMID: 26912360
- Disordered methionine metabolism in MTAP/CDKN2A-deleted cancers leads to dependence on PRMT5. | Mavrakis, KJ., et al. 2016. Science. 351: 1208-13. PMID: 26912361
- PRMT5 control of cGAS/STING and NLRC5 pathways defines melanoma response to antitumor immunity. | Kim, H., et al. 2020. Sci Transl Med. 12: PMID: 32641491
- PRMT5 function and targeting in cancer. | Kim, H. and Ronai, ZA. 2020. Cell Stress. 4: 199-215. PMID: 32743345
- PRMT5 inhibition disrupts splicing and stemness in glioblastoma. | Sachamitr, P., et al. 2021. Nat Commun. 12: 979. PMID: 33579912
- PRMT5 activates AKT via methylation to promote tumor metastasis. | Huang, L., et al. 2022. Nat Commun. 13: 3955. PMID: 35803962
- PRMT5 methylating SMAD4 activates TGF-β signaling and promotes colorectal cancer metastasis. | Liu, A., et al. 2023. Oncogene. 42: 1572-1584. PMID: 36991117
- PRMT5 reduces immunotherapy efficacy in triple-negative breast cancer by methylating KEAP1 and inhibiting ferroptosis. | Wang, Z., et al. 2023. J Immunother Cancer. 11: PMID: 37380368
- MRTX1719 Is an MTA-Cooperative PRMT5 Inhibitor That Exhibits Synthetic Lethality in Preclinical Models and Patients with MTAP-Deleted Cancer. | Engstrom, LD., et al. 2023. Cancer Discov. 13: 2412-2431. PMID: 37552839
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRMT5 Antibody (PRMT5-21) | sc-59650 | 100 µg/ml | $316.00 |