PRMT5 Antibody (A-11): sc-376937

PRMT5 Antibody (A-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects PRMT5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-PRMT5 antibody (A-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PRMT5 plays a crucial role in regulating gene expression and cell proliferation through its function as a type II protein-arginine methyltransferase, which catalyzes symmetrical dimethylation of arginine residues on target proteins. This post-translational modification is essential for spliceosome assembly, a complex responsible for processing pre-mRNA into mature mRNA, as PRMT5 facilitates binding of Sm proteins to small nuclear RNAs (snRNAs). Methylation of Sm proteins by PRMT5 enhances stability and function, thereby influencing splicing processes and impacting gene expression. PRMT5 is predominantly localized in cytoplasm across various tissues and associates with transcription start site region of cyclin E1 promoter, linking PRMT5 to cell cycle progression regulation. Human PRMT5 gene is located on chromosome 14q11, highlighting significance in cellular processes and potential implications in cancer biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PRMT5 Antibody (A-11) References:

1. The human homologue of the yeast proteins Skb1 and Hsl7p interacts with Jak kinases and contains protein methyltransferase activity.  |  Pollack, BP., et al. 1999. J Biol Chem. 274: 31531-42. PMID: 10531356
2. PRMT3 is a distinct member of the protein arginine N-methyltransferase family. Conferral of substrate specificity by a zinc-finger domain.  |  Frankel, A. and Clarke, S. 2000. J Biol Chem. 275: 32974-82. PMID: 10931850
3. Prmt5, which forms distinct homo-oligomers, is a member of the protein-arginine methyltransferase family.  |  Rho, J., et al. 2001. J Biol Chem. 276: 11393-401. PMID: 11152681
4. PRMT5 (Janus kinase-binding protein 1) catalyzes the formation of symmetric dimethylarginine residues in proteins.  |  Branscombe, TL., et al. 2001. J Biol Chem. 276: 32971-6. PMID: 11413150
5. The methylosome, a 20S complex containing JBP1 and pICln, produces dimethylarginine-modified Sm proteins.  |  Friesen, WJ., et al. 2001. Mol Cell Biol. 21: 8289-300. PMID: 11713266
6. Methylation of Sm proteins by a complex containing PRMT5 and the putative U snRNP assembly factor pICln.  |  Meister, G., et al. 2001. Curr Biol. 11: 1990-4. PMID: 11747828
7. Negative regulation of transcription by the type II arginine methyltransferase PRMT5.  |  Fabbrizio, E., et al. 2002. EMBO Rep. 3: 641-5. PMID: 12101096
8. A protein methyl transferase, PRMT5, selectively blocks oncogenic ras-p21 mitogenic signal transduction.  |  Chie, L., et al. 2003. Ann Clin Lab Sci. 33: 200-7. PMID: 12817625
9. MTAP Deletions in Cancer Create Vulnerability to Targeting of the MAT2A/PRMT5/RIOK1 Axis.  |  Marjon, K., et al. 2016. Cell Rep. 15: 574-587. PMID: 27068473
10. PRMT5 disruption drives antitumor immunity in cervical cancer by reprogramming T cell-mediated response and regulating PD-L1 expression.  |  Jiang, Y., et al. 2021. Theranostics. 11: 9162-9176. PMID: 34522232
11. Epigenetic regulation of vascular smooth muscle cell phenotypic switch and neointimal formation by PRMT5.  |  Zhu, N., et al. 2023. Cardiovasc Res. 119: 2244-2255. PMID: 37486354
12. PRMT5-mediated FUBP1 methylation accelerates prostate cancer progression.  |  Yan, W., et al. 2024. J Clin Invest. 134: PMID: 39146021

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PRMT5 Antibody (A-11) has 43 citations in a variety of scientific publications.

Primary antibodies like Anti-PRMT5 Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PRMT5 Antibody (A-11) Specifications

Alternate Product Name: PRMT5

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 338-637

Epitope Target: PRMT5

Epitope Species: human

Product Reactivity/Detection: PRMT5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, bovine, porcine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 43 citations in various scientific publications

Available Conjugates/Forms for PRMT5 Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin