PRMT1 Antibody (B-2): sc-166963

PRMT1 Antibody (B-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects PRMT1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PRMT1 Antibody (B-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PRMT1, a member of the type 1 protein arginine N-methyltransferase family, plays a crucial role in post-translational modification of RNA-binding proteins, essential for regulating gene expression and RNA processing. Located predominantly in the nucleus, PRMT1 is involved in methylation of arginine residues on target proteins, influencing function and interactions. PRMT1 interacts with Interleukin enhancer-binding factor 3 (ILF3) at its carboxy terminus, which modulates PRMT1 activity and highlights the importance of protein-protein interactions in cellular regulation. Alternative mRNA splicing of the PRMT gene results in three isoforms of PRMT1, all retaining enzymatic activity, underscoring the complexity and versatility in various biological contexts. PRMT8, a distinct member of the type 1 PRMT family, exhibits tissue-specific expression and localizes to the plasma membrane, particularly in the brain, where PRMT8 may play a role in neuronal differentiation. PRMT8′s close relationship with PRMT1, including the ability to heterodimerize and share substrate preferences, emphasizes the interconnectedness in cellular signaling pathways.

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PRMT1 Antibody (B-2) References:

1. Skeletal muscle-specific Prmt1 deletion causes muscle atrophy via deregulation of the PRMT6-FOXO3 axis.  |  Choi, S., et al. 2019. Autophagy. 15: 1069-1081. PMID: 30653406
2. Methylation of EZH2 by PRMT1 regulates its stability and promotes breast cancer metastasis.  |  Li, Z., et al. 2020. Cell Death Differ. 27: 3226-3242. PMID: 32895488
3. PRMT1-mediated H4R3me2a recruits SMARCA4 to promote colorectal cancer progression by enhancing EGFR signaling.  |  Yao, B., et al. 2021. Genome Med. 13: 58. PMID: 33853662
4. PHGDH arginine methylation by PRMT1 promotes serine synthesis and represents a therapeutic vulnerability in hepatocellular carcinoma.  |  Wang, K., et al. 2023. Nat Commun. 14: 1011. PMID: 36823188
5. Targeting PRMT1-mediated SRSF1 methylation to suppress oncogenic exon inclusion events and breast tumorigenesis.  |  Li, WJ., et al. 2023. Cell Rep. 42: 113385. PMID: 37938975
6. PRMT1 Inhibition Activates the Interferon Pathway to Potentiate Antitumor Immunity and Enhance Checkpoint Blockade Efficacy in Melanoma.  |  Tao, H., et al. 2024. Cancer Res. 84: 419-433. PMID: 37991725
7. PRMT1 Sustains De Novo Fatty Acid Synthesis by Methylating PHGDH to Drive Chemoresistance in Triple-Negative Breast Cancer.  |  Yamamoto, T., et al. 2024. Cancer Res. 84: 1065-1083. PMID: 38383964
8. PRMT1-mediated PGK1 arginine methylation promotes colorectal cancer glycolysis and tumorigenesis.  |  Liu, H., et al. 2024. Cell Death Dis. 15: 170. PMID: 38402202
9. PRMT1 promotes pancreatic cancer development and resistance to chemotherapy.  |  Ku, B., et al. 2024. Cell Rep Med. 5: 101461. PMID: 38460517
10. CRISPR screening identifies PRMT1 as a key pro-ferroptotic gene via a two-layer regulatory mechanism.  |  Zhang, X., et al. 2024. Cell Rep. 43: 114662. PMID: 39178116

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PRMT1 Antibody (B-2) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-PRMT1 Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PRMT1 Antibody (B-2) Specifications

Alternate Product Name: PRMT1

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 166-300

Epitope Target: PRMT1

Epitope Species: human

Product Reactivity/Detection: PRMT1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for PRMT1 Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred PRMT1 antibody is PRMT1 Antibody (PRMT1-171): sc-59648.