PREPL Antibody (E-9): sc-393321
- PREPL Antibody (E-9) is a mouse monoclonal IgG1 κ PREPL antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 512-727 mapping at the C-terminus of PREPL of human origin
- PREPL Antibody (E-9) is recommended for detection of PREPL of human origin by WB, IP, IF and ELISA
- Anti-PREPL Antibody (E-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PREPL Antibody (E-9) for WB applications. These reagents are now offered in bundles with PREPL Antibody (E-9) (see ordering information below).
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PREPL Antibody (E-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects PREPL protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PREPL (E-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PREPL, or Prolyl endopeptidase-like, is a 727 amino acid cytosolic protein that plays a crucial role in regulating peptide metabolism and is part of the prolyl oligopeptidase subfamily of serine peptidases. PREPL functions as a homodimer and exhibits widespread expression, with highest levels found in heart, brain, kidney, and skeletal muscle. A unique mechanism of action, which relies solely on the carboxy-terminal domain for activity, distinguishes PREPL from other family members like prolyl endopeptidase and oligopeptidase B, which require both amino and carboxy-terminal sequences. Defects in the gene encoding PREPL are linked to hypotonia-cystinuria syndrome, also known as 2p21 deletion syndrome, characterized by hypotonia at birth, growth hormone deficiency, and failure to thrive due to homozygous deletions affecting both SLC3A1 and PREPL genes on chromosome 2p21. Alternative splicing of PREPL gene results in production of four distinct isoforms, further underscoring PREPL′s complexity and significance in human health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PREPL Antibody (E-9) References:
- The 2p21 deletion syndrome: characterization of the transcription content. | Parvari, R., et al. 2005. Genomics. 86: 195-211. PMID: 15913950
- The PREPL A protein, a new member of the prolyl oligopeptidase family, lacking catalytic activity. | Szeltner, Z., et al. 2005. Cell Mol Life Sci. 62: 2376-81. PMID: 16143824
- Deletion of PREPL, a gene encoding a putative serine oligopeptidase, in patients with hypotonia-cystinuria syndrome. | Jaeken, J., et al. 2006. Am J Hum Genet. 78: 38-51. PMID: 16385448
- PREPL: a putative novel oligopeptidase propelled into the limelight. | Martens, K., et al. 2006. Biol Chem. 387: 879-83. PMID: 16913837
- Deletion of C2orf34, PREPL and SLC3A1 causes atypical hypotonia-cystinuria syndrome. | Chabrol, B., et al. 2008. J Med Genet. 45: 314-8. PMID: 18234729
- Multi-system disorder syndromes associated with cystinuria type I. | Martens, K., et al. 2008. Curr Mol Med. 8: 544-50. PMID: 18781961
- Cooperation between NRF-2 and YY-1 transcription factors is essential for triggering the expression of the PREPL-C2ORF34 bidirectional gene pair. | Huang, CC. and Chang, WS. 2009. BMC Mol Biol. 10: 67. PMID: 19575798
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PREPL Antibody (E-9) | sc-393321 | 200 µg/ml | $316.00 | |||
PREPL Antibody (E-9): m-IgG Fc BP-HRP Bundle | sc-530429 | 200 µg Ab; 10 µg BP | $354.00 | |||
PREPL Antibody (E-9): m-IgGκ BP-HRP Bundle | sc-523903 | 200 µg Ab, 40 µg BP | $354.00 | |||
PREPL Antibody (E-9) AC | sc-393321 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PREPL Antibody (E-9) HRP | sc-393321 HRP | 200 µg/ml | $316.00 | |||
PREPL Antibody (E-9) FITC | sc-393321 FITC | 200 µg/ml | $330.00 | |||
PREPL Antibody (E-9) PE | sc-393321 PE | 200 µg/ml | $343.00 | |||
PREPL Antibody (E-9) Alexa Fluor® 488 | sc-393321 AF488 | 200 µg/ml | $357.00 | |||
PREPL Antibody (E-9) Alexa Fluor® 546 | sc-393321 AF546 | 200 µg/ml | $357.00 | |||
PREPL Antibody (E-9) Alexa Fluor® 594 | sc-393321 AF594 | 200 µg/ml | $357.00 | |||
PREPL Antibody (E-9) Alexa Fluor® 647 | sc-393321 AF647 | 200 µg/ml | $357.00 | |||
PREPL Antibody (E-9) Alexa Fluor® 680 | sc-393321 AF680 | 200 µg/ml | $357.00 | |||
PREPL Antibody (E-9) Alexa Fluor® 790 | sc-393321 AF790 | 200 µg/ml | $357.00 |