PREP Antibody (C-12): sc-365416
- PREP Antibody (C-12) is a mouse monoclonal IgG1 κ PREP antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of PREP (Prolyl endopeptidase) of human origin
- PREP Antibody (C-12) is recommended for detection of PREP of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PREP Antibody (C-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for PREP Antibody (C-12) for WB applications. This reagent is now offered in a bundle with PREP Antibody (C-12) (see ordering information below).
PREP Antibody (C-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects PREP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PREP antibody (C-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PREP protein, also known as prolyl endopeptidase or PEP, is a 710 amino acid enzyme that resides in the cytoplasm and is classified within the peptidase S9A family. PREP protein plays a crucial role in the cleavage of peptide bonds on the C-terminal side of prolyl residues, specifically within proteins that are approximately 30 amino acids long. PREP enzymatic activity is vital for the processing of neuropeptides and hormones, which are essential for various signaling and regulatory pathways throughout the body. PREP gene is located on human chromosome 6, a region implicated in several health conditions, including early onset intestinal cancer and various neurodegenerative disorders. PREP interactions with other proteins can influence the balance of neuropeptide signaling, highlighting its importance in maintaining physiological homeostasis and potential role in disease mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PREP Antibody (C-12) References:
- Pyroglutamyl peptidase I and prolyl endopeptidase in human semen: increased activity in necrozoospermia. | Valdivia, A., et al. 2004. Regul Pept. 122: 79-84. PMID: 15380924
- Subcellular localization suggests novel functions for prolyl endopeptidase in protein secretion. | Schulz, I., et al. 2005. J Neurochem. 94: 970-9. PMID: 16092940
- Prolyl endopeptidase cleaves the apoptosis rescue peptide humanin and exhibits an unknown post-cysteine cleavage specificity. | Bär, JW., et al. 2006. Adv Exp Med Biol. 575: 103-8. PMID: 16700513
- No association between the PREP gene and lithium responsive bipolar disorder. | Mamdani, F., et al. 2007. BMC Psychiatry. 7: 9. PMID: 17324276
- Distribution of immunoreactive prolyl oligopeptidase in human and rat brain. | Myöhänen, TT., et al. 2007. Neurochem Res. 32: 1365-74. PMID: 17401647
- Deficient activity of mammalian prolyl oligopeptidase on the immunoactive peptide digestion in coeliac disease. | Garcia-Horsman, JA., et al. 2007. Scand J Gastroenterol. 42: 562-71. PMID: 17454876
- Prolyl endopeptidase. | Wilk, S. 1983. Life Sci. 33: 2149-57. PMID: 6358755
- Molecular cloning and characterization of prolyl endopeptidase from human T cells. | Shirasawa, Y., et al. 1994. J Biochem. 115: 724-9. PMID: 8089089
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PREP Antibody (C-12) | sc-365416 | 200 µg/ml | $322.00 | |||
PREP Antibody (C-12): m-IgG1 BP-HRP Bundle | sc-531707 | 200 µg Ab; 20 µg BP | $361.00 | |||
PREP Antibody (C-12) AC | sc-365416 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PREP Antibody (C-12) HRP | sc-365416 HRP | 200 µg/ml | $322.00 | |||
PREP Antibody (C-12) FITC | sc-365416 FITC | 200 µg/ml | $336.00 | |||
PREP Antibody (C-12) PE | sc-365416 PE | 200 µg/ml | $349.00 | |||
PREP Antibody (C-12) Alexa Fluor® 488 | sc-365416 AF488 | 200 µg/ml | $364.00 | |||
PREP Antibody (C-12) Alexa Fluor® 546 | sc-365416 AF546 | 200 µg/ml | $364.00 | |||
PREP Antibody (C-12) Alexa Fluor® 594 | sc-365416 AF594 | 200 µg/ml | $364.00 | |||
PREP Antibody (C-12) Alexa Fluor® 647 | sc-365416 AF647 | 200 µg/ml | $364.00 | |||
PREP Antibody (C-12) Alexa Fluor® 680 | sc-365416 AF680 | 200 µg/ml | $364.00 | |||
PREP Antibody (C-12) Alexa Fluor® 790 | sc-365416 AF790 | 200 µg/ml | $364.00 |