Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
prefoldin 3 CRISPR/Cas9 KO Plasmid (m): sc-423658
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- prefoldin 3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the prefoldin 3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: prefoldin 3 Antibody (D-5): sc-390524
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
prefoldin 3 CRISPR/Cas9 KO Plasmid (m) | sc-423658 | 20 µg | $397.00 |
Overview
Vbp1 encodes prefoldin 3, a cochaperone component of the prefoldin-like machinery that supports cytosolic protein homeostasis by assisting the folding and stabilization of select client proteins. Prefoldin 3 contributes to proteostasis networks linked to cytoskeletal organization, macromolecular complex assembly, and coordination with chaperone pathways that limit protein misfolding under cellular stress. Through these processes, Vbp1 activity can influence cell-cycle progression, stress adaptation, and the balance between protein folding and degradation. Dysregulation of chaperone-associated pathways is relevant to models of proteotoxic stress and neurodegeneration, where altered folding capacity and quality control can modify disease-associated phenotypes.
prefoldin 3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Vbp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Vbp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Vbp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish prefoldin 3 protein expression.
This CRISPR knockout system enables efficient generation of Vbp1-deficient cell models for investigation of prefoldin 3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Vbp1 exon(s) critical for prefoldin 3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Vbp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by prefoldin 3 CRISPR/Cas9 KO Plasmid (m) and prefoldin 3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Vbp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by prefoldin 3 HDR Plasmid (m) and prefoldin 3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Vbp1 homology arms to support homology-directed repair at defined Vbp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.