prefoldin 3 Antibody (D-5): sc-390524

Prefoldin 3 Antibody (D-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects prefoldin 3 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-prefoldin 3 antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Prefoldin 3 also known as VHL binding protein-1 (VBP1), plays a crucial role in cellular stress response by facilitating proper protein folding, which is essential for maintaining cellular homeostasis and function. Prefoldin 3 is primarily located in the cytoplasm and is involved in transporting unfolded proteins to the cytosolic chaperonin complex, where proper folding occurs. Prefoldin 3 forms complexes with the von Hippel-Lindau (VHL) protein, highlighting a significant role in regulating hypoxia-inducible factors and influencing cellular responses to oxygen levels. Prefoldin 3 translocates from perinuclear granules to the nucleus or cytoplasm, indicating dynamic involvement in cellular processes. Prefoldin 3 versatility in protein interactions underscores importance in various signaling pathways and cellular functions, making Prefoldin 3 monoclonal antibody (D-5) a valuable target for research in protein folding and cellular stress responses.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

prefoldin 3 Antibody (D-5) References:

1. Genomic organization and chromosomal localization of the human CUL2 gene and the role of von Hippel-Lindau tumor suppressor-binding protein (CUL2 and VBP1) mutation and loss in renal-cell carcinoma development.  |  Clifford, SC., et al. 1999. Genes Chromosomes Cancer. 26: 20-8. PMID: 10441001
2. VBP1 facilitates proteasome and autophagy-mediated degradation of MutS homologue hMSH4.  |  Xu, Y. and Her, C. 2013. FASEB J. 27: 4799-810. PMID: 23964080
3. VBP1 represses cancer metastasis by enhancing HIF-1α degradation induced by pVHL.  |  Kim, JA., et al. 2018. FEBS J. 285: 115-126. PMID: 29121446
4. VBP1 modulates Wnt/β-catenin signaling by mediating the stability of the transcription factors TCF/LEFs.  |  Zhang, H., et al. 2020. J Biol Chem. 295: 16826-16839. PMID: 32989053
5. VBP1 negatively regulates CHIP and selectively inhibits the activity of hypoxia-inducible factor (HIF)-1α but not HIF-2α.  |  Yue, Y., et al. 2023. J Biol Chem. 299: 104829. PMID: 37201586
6. VBP1 promotes tumor proliferation as a part of the hypoxia-related signature in esophageal squamous cell carcinoma.  |  Miao, H., et al. 2024. Hum Cell. 37: 1141-1155. PMID: 38700744
7. Late stage melanoma is hallmarked by low NLGN4X expression leading to HIF1A accumulation.  |  Schörghofer, D., et al. 2024. Br J Cancer. 131: 468-480. PMID: 38902533
8. Identification of a novel protein (VBP-1) binding to the von Hippel-Lindau (VHL) tumor suppressor gene product.  |  Tsuchiya, H., et al. 1996. Cancer Res. 56: 2881-5. PMID: 8674032
9. Characterization of the gene (VBP1) and transcript for the von Hippel-Lindau binding protein and isolation of the highly conserved murine homologue.  |  Brinke, A., et al. 1997. Genomics. 45: 105-12. PMID: 9339366
10. Prefoldin, a chaperone that delivers unfolded proteins to cytosolic chaperonin.  |  Vainberg, IE., et al. 1998. Cell. 93: 863-73. PMID: 9630229
11. Expression of the von Hippel-Lindau-binding protein-1 (Vbp1) in fetal and adult mouse tissues.  |  Hemberger, M., et al. 1999. Hum Mol Genet. 8: 229-36. PMID: 9931330

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-prefoldin 3 Antibody (D-5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-prefoldin 3 Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

prefoldin 3 Antibody (D-5) Specifications

Alternate Product Name: prefoldin 3

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-92

Epitope Target: prefoldin 3

Epitope Species: human

Product Reactivity/Detection: prefoldin 3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for prefoldin 3 Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin