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prefoldin 3 Antibody (B-3): sc-390465

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Datasheets
  • prefoldin 3 Antibody (B-3) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
  • raised against amino acids 1-92 mapping at the N-terminus of prefoldin 3 of human origin
  • recommended for detection of prefoldin 3 of mouse, rat and human origin by WB, IP, IF and ELISA
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for prefoldin 3 Antibody (B-3) for WB applications. These reagents are now offered in bundles with prefoldin 3 Antibody (B-3) (see ordering information below).
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Prefoldin 3 Antibody (B-3) is a mouse monoclonal IgG1 antibody that detects prefoldin 3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Prefoldin 3, also known as VHL binding protein-1, plays a crucial role in cellular stress response by facilitating proper protein folding, particularly under conditions where misfolding can lead to cellular dysfunction. Prefoldin 3 is primarily located in the cytoplasm and forms complexes with the von Hippel-Lindau (VHL) tumor suppressor protein, which is essential for regulating hypoxia-inducible factors and maintaining cellular oxygen homeostasis. The interaction between prefoldin 3 and VHL is significant because this binding aids in VHL stabilization and influences hypoxia-inducible factor degradation, thereby impacting various cellular processes such as angiogenesis and metabolism. Anti-prefoldin 3 antibody (B-3) serves as an invaluable tool for researchers studying protein folding mechanisms and cellular stress response pathways.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

prefoldin 3 Antibody (B-3) References:

  1. Filamin (FLN1), plexin (SEX), major palmitoylated protein p55 (MPP1), and von-Hippel Lindau binding protein (VBP1) are not involved in incontinentia pigmenti type 2.  |  Aradhya, S., et al. 2000. Am J Med Genet. 94: 79-84. PMID: 10982489
  2. Recurrent inversion breaking intron 1 of the factor VIII gene is a frequent cause of severe hemophilia A.  |  Bagnall, RD., et al. 2002. Blood. 99: 168-74. PMID: 11756167
  3. Interaction between Mnk2 and CBC(VHL) ubiquitin ligase E3 complex.  |  Wang, P., et al. 2006. Sci China C Life Sci. 49: 265-73. PMID: 16856496
  4. VBP15: preclinical characterization of a novel anti-inflammatory delta 9,11 steroid.  |  Reeves, EKM., et al. 2013. Bioorg Med Chem. 21: 2241-2249. PMID: 23498916
  5. Global comparison of chromosome X genes of pulmonary telocytes with mesenchymal stem cells, fibroblasts, alveolar type II cells, airway epithelial cells, and lymphocytes.  |  Zhu, Y., et al. 2015. J Transl Med. 13: 318. PMID: 26416664
  6. Expression of Agrobacterium Homolog Genes Encoding T-complex Recruiting Protein under Virulence Induction Conditions.  |  Yang, J., et al. 2015. Front Microbiol. 6: 1379. PMID: 26696988
  7. Differential HDAC1/2 network analysis reveals a role for prefoldin/CCT in HDAC1/2 complex assembly.  |  Banks, CAS., et al. 2018. Sci Rep. 8: 13712. PMID: 30209338
  8. Identification of microRNA editing sites in three subtypes of leukemia.  |  Xie, W., et al. 2022. Front Mol Biosci. 9: 1014288. PMID: 36452459
  9. Cuproptosis-related genes prediction feature and immune microenvironment in major depressive disorder.  |  Lei, D., et al. 2023. Heliyon. 9: e18497. PMID: 37576193
  10. Contribution of Rare and Potentially Functionally Relevant Sequence Variants in Schizophrenia Risk-Locus Xq28,distal.  |  Claus, I., et al. 2024. Am J Med Genet B Neuropsychiatr Genet. e33011. PMID: 39473393

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

prefoldin 3 Antibody (B-3)

sc-390465
200 µg/ml
$322.00

prefoldin 3 Antibody (B-3): m-IgG Fc BP-HRP Bundle

sc-540804
200 µg Ab; 10 µg BP
$361.00

prefoldin 3 Antibody (B-3): m-IgG1 BP-HRP Bundle

sc-542379
200 µg Ab; 20 µg BP
$361.00

How much sample do you recommend loading on the gel for Western blot detection using prefoldin 3 (B-3): sc-390465?

Asked by: Trav11
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
  • y_2026, m_4, d_23, h_7CST
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Rated 5 out of 5 by from Produced positive Western blot data of prefoldinProduced positive Western blot data of prefoldin 3 expression in HL-60 and Hep G2 whole cell lysates. -SCBT QC
Date published: 2013-05-18
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prefoldin 3 Antibody (B-3) is rated 5.0 out of 5 by 1.
  • y_2026, m_4, d_23, h_7
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