Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PRDM8 CRISPR/Cas9 KO Plasmid (h): sc-406359
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRDM8 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PRDM8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PRDM8 Antibody (E-3): sc-390001
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRDM8 CRISPR/Cas9 KO Plasmid (h) | sc-406359 | 20 µg | $397.00 |
Overview
PRDM8 (PR/SET domain 8) encodes a PR/SET domain-containing transcriptional regulator that influences chromatin state and gene expression programs during development. As a member of the PRDM family, PRDM8 is linked to epigenetic control mechanisms that shape neuronal differentiation, axon guidance, and broader lineage-specific transcriptional networks. Altered regulation of PRDM8-associated pathways can perturb cell fate decisions and has been investigated in the context of neurodevelopmental dysfunction and transcriptional dysregulation observed in cancer-relevant epigenetic landscapes. Studying PRDM8 supports mechanistic dissection of chromatin-mediated repression/activation, enhancer–promoter control, and downstream effects on proliferation and differentiation.
PRDM8 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PRDM8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PRDM8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PRDM8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PRDM8 protein expression.
This CRISPR knockout system enables efficient generation of PRDM8-deficient cell models for investigation of PRDM8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PRDM8 exon(s) critical for PRDM8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PRDM8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PRDM8 CRISPR/Cas9 KO Plasmid (h) and PRDM8 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PRDM8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PRDM8 HDR Plasmid (h) and PRDM8 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PRDM8 homology arms to support homology-directed repair at defined PRDM8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.