PRDM5 Antibody (A-12): sc-376277
- PRDM5 Antibody (A-12) is a mouse monoclonal IgG1 κ PRDM5 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 53-175 mapping at the N-terminus of PRDM5 of human origin
- PRDM5 Antibody (A-12) is recommended for detection of PRDM5 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PRDM5 Antibody (A-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-376277 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PRDM5 Antibody (A-12) for WB applications. These reagents are now offered in bundles with PRDM5 Antibody (A-12) (see ordering information below).
PRDM5 Antibody (A-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects PRDM5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PRDM5 Antibody (A-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PRDM5 plays a crucial role in regulating gene expression and is involved in cellular processes such as differentiation and apoptosis, making PRDM5 significant in cancer biology. PRDM5 is located in the nucleus, where PRDM5 functions as a transcriptional regulator, influencing the expression of genes associated with tumor suppression. Nuclear localization allows PRDM5 to interact with other nuclear proteins and DNA, thereby modulating pathways that can prevent tumorigenesis. PRDM5 has been linked to the regulation of genes silenced in various cancers, including breast, ovarian, and liver cancers, highlighting PRDM5′s potential as a therapeutic target. The gene encoding PRDM5, consisting of 630 amino acids and featuring a PR domain followed by 16 zinc finger motifs, is mapped to human chromosome 4q27, a region associated with several tumor suppressor genes. PRDM5′s ability to induce G2/M arrest and apoptosis in tumor cells upon adenoviral expression further underscores PRDM5′s importance in cancer research and potential therapeutic applications.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PRDM5 Antibody (A-12) References:
- PFM1 (PRDM4), a new member of the PR-domain family, maps to a tumor suppressor locus on human chromosome 12q23-q24.1. | Yang, XH. and Huang, S. 1999. Genomics. 61: 319-25. PMID: 10552934
- The yin-yang of PR-domain family genes in tumorigenesis. | Jiang, GL. and Huang, S. 2000. Histol Histopathol. 15: 109-17. PMID: 10668202
- PR domain of rous sarcoma virus Gag causes an assembly/budding defect in insect cells. | Johnson, MC., et al. 2001. J Virol. 75: 4407-12. PMID: 11287591
- A novel EVI1 gene family, MEL1, lacking a PR domain (MEL1S) is expressed mainly in t(1;3)(p36;q21)-positive AML and blocks G-CSF-induced myeloid differentiation. | Nishikata, I., et al. 2003. Blood. 102: 3323-32. PMID: 12816872
- PRDM5 is silenced in human cancers and has growth suppressive activities. | Deng, Q. and Huang, S. 2004. Oncogene. 23: 4903-10. PMID: 15077163
- Oligomerization of Evi-1 regulated by the PR domain contributes to recruitment of corepressor CtBP. | Nitta, E., et al. 2005. Oncogene. 24: 6165-73. PMID: 15897867
- The retinoblastoma interacting zinc finger gene RIZ produces a PR domain-lacking product through an internal promoter. | Liu, L., et al. 1997. J Biol Chem. 272: 2984-91. PMID: 9006946
- Transcriptional repression mediated by the PR domain zinc finger gene RIZ. | Xie, M., et al. 1997. J Biol Chem. 272: 26360-6. PMID: 9334209
- The PR domain of the Rb-binding zinc finger protein RIZ1 is a protein binding interface and is related to the SET domain functioning in chromatin-mediated gene expression. | Huang, S., et al. 1998. J Biol Chem. 273: 15933-9. PMID: 9632640
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRDM5 Antibody (A-12) | sc-376277 | 200 µg/ml | $316.00 | |||
PRDM5 Antibody (A-12): m-IgG Fc BP-HRP Bundle | sc-527519 | 200 µg Ab; 10 µg BP | $354.00 | |||
PRDM5 Antibody (A-12): m-IgG1 BP-HRP Bundle | sc-532892 | 200 µg Ab; 20 µg BP | $354.00 | |||
PRDM5 Antibody (A-12) X | sc-376277 X | 200 µg/0.1 ml | $316.00 | |||
PRDM5 Antibody (A-12) AC | sc-376277 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PRDM5 Antibody (A-12) HRP | sc-376277 HRP | 200 µg/ml | $316.00 | |||
PRDM5 Antibody (A-12) FITC | sc-376277 FITC | 200 µg/ml | $330.00 | |||
PRDM5 Antibody (A-12) PE | sc-376277 PE | 200 µg/ml | $343.00 | |||
PRDM5 Antibody (A-12) Alexa Fluor® 488 | sc-376277 AF488 | 200 µg/ml | $357.00 | |||
PRDM5 Antibody (A-12) Alexa Fluor® 546 | sc-376277 AF546 | 200 µg/ml | $357.00 | |||
PRDM5 Antibody (A-12) Alexa Fluor® 594 | sc-376277 AF594 | 200 µg/ml | $357.00 | |||
PRDM5 Antibody (A-12) Alexa Fluor® 647 | sc-376277 AF647 | 200 µg/ml | $357.00 | |||
PRDM5 Antibody (A-12) Alexa Fluor® 680 | sc-376277 AF680 | 200 µg/ml | $357.00 | |||
PRDM5 Antibody (A-12) Alexa Fluor® 790 | sc-376277 AF790 | 200 µg/ml | $357.00 |