PRAT4A Antibody (F-6): sc-515151
- PRAT4A Antibody (F-6) is a mouse monoclonal IgG1 κ PRAT4A antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 36-139 mapping near the N-terminus of PRAT4A of human origin
- PRAT4A Antibody (F-6) is recommended for detection of PRAT4A of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PRAT4A Antibody (F-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for PRAT4A Antibody (F-6) for WB applications. This reagent is now offered in a bundle with PRAT4A Antibody (F-6) (see ordering information below).
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PRAT4A Antibody (F-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects PRAT4A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PRAT4A (F-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PRAT4A protein, also known as CAG4A or TNRC5, plays a crucial role in regulating Toll-like receptor 4 (TLR4) expression on the cell surface, which is essential for the immune system′s ability to recognize and respond to microbial threats. TLR4 is a key player in the innate immune response, specifically in detecting lipopolysaccharide (LPS), a potent endotoxin that triggers immune activation in cells such as macrophages and dendritic cells. The interaction between PRAT4A and TLR4 is vital, as PRAT4A facilitates proper localization and expression of TLR4 on the cell surface, thereby enhancing the immune response to pathogens. PRAT4A is a 278 amino acid protein that exists in two isoforms due to alternative splicing, highlighting complexity and potential regulatory roles in immune signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PRAT4A Antibody (F-6) References:
- Cutting edge: cell surface expression and lipopolysaccharide signaling via the toll-like receptor 4-MD-2 complex on mouse peritoneal macrophages. | Akashi, S., et al. 2000. J Immunol. 164: 3471-5. PMID: 10725698
- Toll-like receptor family and signalling pathway. | Muzio, M., et al. 2000. Biochem Soc Trans. 28: 563-6. PMID: 11044375
- TLR2: cellular sensor for microbial and endogenous molecular patterns. | Kirschning, CJ. and Schumann, RR. 2002. Curr Top Microbiol Immunol. 270: 121-44. PMID: 12467248
- Identification of mouse MD-2 residues important for forming the cell surface TLR4-MD-2 complex recognized by anti-TLR4-MD-2 antibodies, and for conferring LPS and taxol responsiveness on mouse TLR4 by alanine-scanning mutagenesis. | Kawasaki, K., et al. 2003. J Immunol. 170: 413-20. PMID: 12496426
- A molecule that is associated with Toll-like receptor 4 and regulates its cell surface expression. | Konno, K., et al. 2006. Biochem Biophys Res Commun. 339: 1076-82. PMID: 16338228
- A protein associated with toll-like receptor 4 (PRAT4A) regulates cell surface expression of TLR4. | Wakabayashi, Y., et al. 2006. J Immunol. 177: 1772-9. PMID: 16849487
- Mechanism regulating cell surface expression and activation of Toll-like receptor 4. | Saitoh, S. and Miyake, K. 2006. Chem Rec. 6: 311-9. PMID: 17304555
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRAT4A Antibody (F-6) | sc-515151 | 200 µg/ml | $322.00 | |||
PRAT4A Antibody (F-6): m-IgG Fc BP-HRP Bundle | sc-552126 | 200 µg Ab; 10 µg BP | $361.00 | |||
PRAT4A Antibody (F-6) AC | sc-515151 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PRAT4A Antibody (F-6) HRP | sc-515151 HRP | 200 µg/ml | $322.00 | |||
PRAT4A Antibody (F-6) FITC | sc-515151 FITC | 200 µg/ml | $336.00 | |||
PRAT4A Antibody (F-6) PE | sc-515151 PE | 200 µg/ml | $349.00 | |||
PRAT4A Antibody (F-6) Alexa Fluor® 488 | sc-515151 AF488 | 200 µg/ml | $364.00 | |||
PRAT4A Antibody (F-6) Alexa Fluor® 546 | sc-515151 AF546 | 200 µg/ml | $364.00 | |||
PRAT4A Antibody (F-6) Alexa Fluor® 594 | sc-515151 AF594 | 200 µg/ml | $364.00 | |||
PRAT4A Antibody (F-6) Alexa Fluor® 647 | sc-515151 AF647 | 200 µg/ml | $364.00 | |||
PRAT4A Antibody (F-6) Alexa Fluor® 680 | sc-515151 AF680 | 200 µg/ml | $364.00 | |||
PRAT4A Antibody (F-6) Alexa Fluor® 790 | sc-515151 AF790 | 200 µg/ml | $364.00 |