PRAME Antibody (H-10): sc-137188
- PRAME Antibody (H-10) is a mouse monoclonal IgG1 κ, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 126-205 mapping within an internal region of PRAME of human origin
- PRAME Antibody (H-10) is recommended for detection of PRAME of human origin by WB, IP, IF and ELISA
- Anti-PRAME Antibody (H-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PRAME Antibody (H-10) for WB applications. These reagents are now offered in bundles with PRAME Antibody (H-10) (see ordering information below).
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SEE ALSO...
PRAME Antibody (H-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects PRAME protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PRAME antibody (H-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The preferentially expressed antigen of melanoma (PRAME) plays a crucial role in tumor immunology, as PRAME is a tumor-associated antigen primarily expressed in cancer cells, with limited expression in normal tissues, making PRAME a promising target for immunotherapy. PRAME is recognized by cytotoxic T lymphocytes and is expressed in a wide range of malignancies, including melanoma, non-small-cell lung carcinoma, sarcomas, head and neck tumors, and renal carcinomas. High PRAME expression in a significant fraction of tumors underscores PRAME′s potential as a therapeutic target, particularly in melanoma, where PRAME is commonly found. PRAME′s ability to elicit an immune response highlights PRAME′s importance in the development of targeted cancer therapies, as PRAME may help to harness the body′s immune system to combat tumor growth effectively.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PRAME Antibody (H-10) References:
- Identification of a new, unorthodox member of the MAGE gene family. | Põld, M., et al. 1999. Genomics. 59: 161-7. PMID: 10409427
- Quantitative monitoring of the PRAME gene for the detection of minimal residual disease in leukaemia. | Matsushita, M., et al. 2001. Br J Haematol. 112: 916-26. PMID: 11298586
- High homogeneity of MAGE, BAGE, GAGE, tyrosinase and Melan-A/MART-1 gene expression in clusters of multiple simultaneous metastases of human melanoma: implications for protocol design of therapeutic antigen-specific vaccination strategies. | Dalerba, P., et al. 1998. Int J Cancer. 77: 200-4. PMID: 9650552
- PRAME, a gene encoding an antigen recognized on a human melanoma by cytolytic T cells, is expressed in acute leukaemia cells. | van Baren, N., et al. 1998. Br J Haematol. 102: 1376-9. PMID: 9753074
- Expression of BAGE, GAGE, and MAGE genes in human gastric carcinoma. | Li, J., et al. 1996. Clin Cancer Res. 2: 1619-25. PMID: 9816341
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRAME Antibody (H-10) | sc-137188 | 200 µg/ml | $316.00 | |||
PRAME Antibody (H-10): m-IgG Fc BP-HRP Bundle | sc-527129 | 200 µg Ab; 10 µg BP | $354.00 | |||
PRAME Antibody (H-10): m-IgG1 BP-HRP Bundle | sc-532502 | 200 µg Ab; 20 µg BP | $354.00 | |||
PRAME Antibody (H-10) AC | sc-137188 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PRAME Antibody (H-10) HRP | sc-137188 HRP | 200 µg/ml | $316.00 | |||
PRAME Antibody (H-10) FITC | sc-137188 FITC | 200 µg/ml | $330.00 | |||
PRAME Antibody (H-10) PE | sc-137188 PE | 200 µg/ml | $343.00 | |||
PRAME Antibody (H-10) Alexa Fluor® 488 | sc-137188 AF488 | 200 µg/ml | $357.00 | |||
PRAME Antibody (H-10) Alexa Fluor® 546 | sc-137188 AF546 | 200 µg/ml | $357.00 | |||
PRAME Antibody (H-10) Alexa Fluor® 594 | sc-137188 AF594 | 200 µg/ml | $357.00 | |||
PRAME Antibody (H-10) Alexa Fluor® 647 | sc-137188 AF647 | 200 µg/ml | $357.00 | |||
PRAME Antibody (H-10) Alexa Fluor® 680 | sc-137188 AF680 | 200 µg/ml | $357.00 | |||
PRAME Antibody (H-10) Alexa Fluor® 790 | sc-137188 AF790 | 200 µg/ml | $357.00 |