PPPDE2 CRISPR/Cas9 KO Plasmid (h): sc-406172
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PPPDE2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PPPDE2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PPPDE2 Antibody (A-6): sc-393863
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PPPDE2 CRISPR/Cas9 KO Plasmid (h) | sc-406172 | 20 µg | $397.00 |
Overview
PPPDE2 (also annotated as DESI1) encodes a cysteine-dependent protease/deubiquitinase-like protein implicated in post-translational regulation of protein stability and signaling. Reported activities include deSUMOylation or ubiquitin-like modifier processing, linking PPPDE2 to proteostasis, stress responses, and dynamic control of nuclear and cytosolic protein function. Through these mechanisms, PPPDE2 may influence pathways governing cell-cycle progression, apoptosis, and transcriptional programs that depend on reversible protein modification. Altered expression or dysregulated modifier turnover has been associated with cellular phenotypes relevant to cancer biology and neurodegenerative processes, supporting its study in disease-relevant model systems.
PPPDE2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DESI1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DESI1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DESI1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PPPDE2 protein expression.
This CRISPR knockout system enables efficient generation of DESI1-deficient cell models for investigation of PPPDE2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DESI1 exon(s) critical for PPPDE2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DESI1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PPPDE2 CRISPR/Cas9 KO Plasmid (h) and PPPDE2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DESI1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PPPDE2 HDR Plasmid (h) and PPPDE2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DESI1 homology arms to support homology-directed repair at defined DESI1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.