Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PPOX CRISPR/Cas9 KO Plasmid (m): sc-422376
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PPOX CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PPOX genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PPOX Antibody (C-12): sc-271768
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PPOX CRISPR/Cas9 KO Plasmid (m) | sc-422376 | 20 µg | $397.00 |
Overview
Mouse Ppox encodes protoporphyrinogen oxidase (PPOX), a mitochondrial inner membrane enzyme that catalyzes the oxidation of protoporphyrinogen IX to protoporphyrin IX in the terminal steps of heme biosynthesis. By controlling flux through the porphyrin pathway, PPOX supports hemoprotein production required for oxidative phosphorylation, cytochrome function, and cellular redox homeostasis. Disruption of PPOX activity can perturb mitochondrial metabolism and elevate porphyrin intermediates, linking this node of heme metabolism to photosensitivity-associated porphyrias and broader oxidative stress phenotypes. Ppox is therefore relevant for studying mitochondrial bioenergetics, erythroid differentiation programs, and metabolic crosstalk between heme availability and reactive oxygen species handling.
PPOX CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ppox gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ppox together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ppox open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PPOX protein expression.
This CRISPR knockout system enables efficient generation of Ppox-deficient cell models for investigation of PPOX signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ppox exon(s) critical for PPOX function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ppox genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PPOX CRISPR/Cas9 KO Plasmid (m) and PPOX CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ppox locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PPOX HDR Plasmid (m) and PPOX HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ppox homology arms to support homology-directed repair at defined Ppox target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.