PPOX Antibody (C-12): sc-271768
- PPOX Antibody (C-12) is a mouse monoclonal IgG2b κ PPOX antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 298-477 mapping at the C-terminus of PPOX of human origin
- PPOX Antibody (C-12) is recommended for detection of PPOX of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PPOX Antibody (C-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PPOX Antibody (C-12) for WB applications. These reagents are now offered in bundles with PPOX Antibody (C-12) (see ordering information below).
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SEE ALSO...
PPOX Antibody (C-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects PPOX protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PPOX (C-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Protoporphyrinogen oxidase (PPOX) plays a crucial role as the penultimate enzyme in the heme biosynthetic pathway, catalyzing the six-electron oxidation of protoporphyrinogen IX to produce protoporphyrin IX, a vital precursor for heme synthesis. Proper functioning of PPOX is essential for maintaining adequate heme levels, which are critical for various biological processes, including oxygen transport and electron transfer in cellular respiration. PPOX protein is localized to the inner membrane of mitochondria across various tissues, such as the heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas, highlighting PPOX importance in energy metabolism and cellular function. Genetic deficiencies in PPOX can lead to variegate porphyria, an autosomal dominant disorder that manifests with cutaneous photosensitivity and neurological symptoms. In rare cases, homozygous variants of this condition result in severe PPOX deficiency, leading to early childhood photosensitization, skeletal abnormalities, and potential cognitive impairments, underscoring PPOX critical role in human health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PPOX Antibody (C-12) References:
- Nine novel mutations in the protoporphyrinogen oxidase gene in Swedish families with variegate porphyria. | Wiman, A., et al. 2003. Clin Genet. 64: 122-30. PMID: 12859407
- Kinetic and physical characterisation of recombinant wild-type and mutant human protoporphyrinogen oxidases. | Maneli, MH., et al. 2003. Biochim Biophys Acta. 1650: 10-21. PMID: 12922165
- Identification of sequences required for the import of human protoporphyrinogen oxidase to mitochondria. | Morgan, RR., et al. 2004. Biochem J. 377: 281-7. PMID: 14535846
- Cloning of a human cDNA for protoporphyrinogen oxidase by complementation in vivo of a hemG mutant of Escherichia coli. | Nishimura, K., et al. 1995. J Biol Chem. 270: 8076-80. PMID: 7713909
- The human protoporphyrinogen oxidase gene (PPOX): organization and location to chromosome 1. | Taketani, S., et al. 1995. Genomics. 29: 698-703. PMID: 8575762
- Protoporphyrinogen oxidase: complete genomic sequence and polymorphisms in the human gene. | Puy, H., et al. 1996. Biochem Biophys Res Commun. 226: 226-30. PMID: 8806618
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PPOX Antibody (C-12) | sc-271768 | 200 µg/ml | $316.00 | |||
PPOX Antibody (C-12): m-IgG Fc BP-HRP Bundle | sc-529257 | 200 µg Ab; 10 µg BP | $354.00 | |||
PPOX Antibody (C-12): m-IgGκ BP-HRP Bundle | sc-522055 | 200 µg Ab, 40 µg BP | $354.00 | |||
PPOX Antibody (C-12) AC | sc-271768 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PPOX Antibody (C-12) HRP | sc-271768 HRP | 200 µg/ml | $316.00 | |||
PPOX Antibody (C-12) FITC | sc-271768 FITC | 200 µg/ml | $330.00 | |||
PPOX Antibody (C-12) PE | sc-271768 PE | 200 µg/ml | $343.00 | |||
PPOX Antibody (C-12) Alexa Fluor® 488 | sc-271768 AF488 | 200 µg/ml | $357.00 | |||
PPOX Antibody (C-12) Alexa Fluor® 546 | sc-271768 AF546 | 200 µg/ml | $357.00 | |||
PPOX Antibody (C-12) Alexa Fluor® 594 | sc-271768 AF594 | 200 µg/ml | $357.00 | |||
PPOX Antibody (C-12) Alexa Fluor® 647 | sc-271768 AF647 | 200 µg/ml | $357.00 | |||
PPOX Antibody (C-12) Alexa Fluor® 680 | sc-271768 AF680 | 200 µg/ml | $357.00 | |||
PPOX Antibody (C-12) Alexa Fluor® 790 | sc-271768 AF790 | 200 µg/ml | $357.00 |