PPAN Antibody (C-7): sc-398273
- PPAN Antibody (C-7) is a mouse monoclonal IgG1 κ PPAN antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 422-445 near the C-terminus of PPAN of human origin
- PPAN Antibody (C-7) is recommended for detection of PPAN of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PPAN Antibody (C-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PPAN Antibody (C-7) for WB applications. These reagents are now offered in bundles with PPAN Antibody (C-7) (see ordering information below).
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PPAN Antibody (C-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects PPAN protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PPAN (C-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PPAN protein, also known as peter pan homolog or Ssf-1 (suppressor of SWI4 1 homolog), is a 473 amino acid protein characterized by one Brix domain and two alternatively spliced isoforms. PPAN is encoded by a gene located on human chromosome 19p13.2 and predominantly localizes in the nucleus, with expression levels highest in heart, skeletal muscle, kidney, and liver tissues. PPAN plays a crucial role in cellular processes, functioning as a putative tumor suppressor in heart failure cells and nontransformed revertants of HeLa cells. PPAN expression is upregulated in myeloid leukemia cells in response to granulocyte-colony stimulating factor and dibutyryl-cAMP, indicating potential involvement in cell growth regulation. Two characteristic CpG islands upstream of PPAN exons suggest multiple promoter regions, which could be significant for regulation and expression in various cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PPAN Antibody (C-7) References:
- Cloning and characterization of peter pan, a novel Drosophila gene required for larval growth. | Migeon, JC., et al. 1999. Mol Biol Cell. 10: 1733-44. PMID: 10359593
- Identification and validation of a gene involved in anchorage-independent cell growth control using a library of randomized hairpin ribozymes. | Welch, PJ., et al. 2000. Genomics. 66: 274-83. PMID: 10873382
- Cloning, genomic organization, and tissue distribution of human Ssf-1. | Suarez-Huerta, N., et al. 2000. Biochem Biophys Res Commun. 275: 37-42. PMID: 10944437
- The black-pearl gene of Drosophila defines a novel conserved protein family and is required for larval growth and survival. | Becker, S., et al. 2001. Gene. 262: 15-22. PMID: 11179663
- Cotranscription and intergenic splicing of human P2Y11 and SSF1 genes. | Communi, D., et al. 2001. J Biol Chem. 276: 16561-6. PMID: 11278528
- The Brix domain protein family -- a key to the ribosomal biogenesis pathway? | Eisenhaber, F., et al. 2001. Trends Biochem Sci. 26: 345-7. PMID: 11406393
- A novel nucleotide receptor in Xenopus activates the cAMP second messenger pathway. | Devader, C., et al. 2007. FEBS Lett. 581: 5332-6. PMID: 17977530
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PPAN Antibody (C-7) | sc-398273 | 200 µg/ml | $322.00 | |||
PPAN Antibody (C-7): m-IgG Fc BP-HRP Bundle | sc-530732 | 200 µg Ab; 10 µg BP | $361.00 | |||
PPAN Antibody (C-7): m-IgGκ BP-HRP Bundle | sc-524266 | 200 µg Ab, 40 µg BP | $361.00 | |||
PPAN Antibody (C-7): m-IgG1 BP-HRP Bundle | sc-544224 | 200 µg Ab; 20 µg BP | $361.00 | |||
PPAN Antibody (C-7) AC | sc-398273 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PPAN Antibody (C-7) HRP | sc-398273 HRP | 200 µg/ml | $322.00 | |||
PPAN Antibody (C-7) FITC | sc-398273 FITC | 200 µg/ml | $336.00 | |||
PPAN Antibody (C-7) PE | sc-398273 PE | 200 µg/ml | $349.00 | |||
PPAN Antibody (C-7) Alexa Fluor® 488 | sc-398273 AF488 | 200 µg/ml | $364.00 | |||
PPAN Antibody (C-7) Alexa Fluor® 546 | sc-398273 AF546 | 200 µg/ml | $364.00 | |||
PPAN Antibody (C-7) Alexa Fluor® 594 | sc-398273 AF594 | 200 µg/ml | $364.00 | |||
PPAN Antibody (C-7) Alexa Fluor® 647 | sc-398273 AF647 | 200 µg/ml | $364.00 | |||
PPAN Antibody (C-7) Alexa Fluor® 680 | sc-398273 AF680 | 200 µg/ml | $364.00 | |||
PPAN Antibody (C-7) Alexa Fluor® 790 | sc-398273 AF790 | 200 µg/ml | $364.00 | |||
PPAN (C-7) Neutralizing Peptide | sc-398273 P | 100 µg/0.5 ml | $69.00 |