PP2A-B56-γ Antibody (E-6): sc-374380

PP2A-B56-γ (E-6) is a mouse monoclonal IgG1 kappa light chain antibody raised against PP2A-B56-gamma (also designated Serine/Threonine phosphatase PP2A-B56 alpha, B56A, PR61A, B56alpha, protein phosphatase 2 regulatory subunit B56 alpha, protein phosphatase 2 (PP2A) B56 alpha, PPP2R5A protein phosphatase 2 regulatory subunit B′alpha, PP2A heterotrimeric (structural A, regulatory B, catalytic C) protein phosphatase, PP2A phosphatase regulatory B56 alpha, protein phosphatase 2 complex, or Ser/Thr phosphatase) that detects PP2A-B56-gamma protein of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP) and enzyme-linked immunosorbent assay (ELISA). PP2A-B56-γ (E-6) antibody is available as both non-conjugated form and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. In eukaryotes, phosphorylation and dephosphorylation of proteins on serine and threonine residues is essential for regulating cellular functions, including division, homeostasis and apoptosis. Protein phosphatases play a crucial role in this process. The protein phosphatase (PP) holoenzyme forms a trimeric complex with regulatory, variable, and catalytic subunits. Four major protein phosphatase catalytic subunit families include PP1, PP2A, PP2B (calcineurin) and PP2C. PPX (also known as PP4) represents a potential new PP family member. PP2A family includes PP2Aα and PP2Aβ subfamily members. PP2A catalytic subunit combines with various regulatory subunits, including PP2A-A-α and -A-β, PP2A-B-α and -B-β, PP2A-C-α and -C-β, PP2A-B56-α, -B56-β, -B56-γ and -B56-δ.

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PP2A-B56-γ Antibody (E-6) References:

1. Structure and expression of two isoforms of the murine calmodulin-dependent protein phosphatase regulatory subunit (calcineurin B).  |  Ueki, K., et al. 1992. Biochem Biophys Res Commun. 187: 537-43. PMID: 1325794
2. Expression and distribution of PPP2R5C gene in leukemia.  |  Zheng, H., et al. 2011. J Hematol Oncol. 4: 21. PMID: 21548944
3. Proliferation inhibition and apoptosis induction of imatinib-resistant chronic myeloid leukemia cells via PPP2R5C down-regulation.  |  Shen, Q., et al. 2013. J Hematol Oncol. 6: 64. PMID: 24004697
4. Growth arrest of PPP2R5C and PPP2R5D double knockout mice indicates a genetic interaction and conserved function for these PP2A B subunits.  |  Dyson, JJ., et al. 2022. FASEB Bioadv. 4: 273-282. PMID: 35415460
5. A recurrent variant in PPP2R5C identified in individuals with macrocephaly, intellectual disability, and seizures.  |  Muir, AM., et al. 2024. HGG Adv. 6: 100394. PMID: 39696819
6. Pathogenic de novo variants in PPP2R5C cause a neurodevelopmental disorder within the Houge-Janssens syndrome spectrum.  |  Verbinnen, I., et al. 2025. Am J Hum Genet.. PMID: 39978342
7. Molecular cloning of NIPP-1, a nuclear inhibitor of protein phosphatase-1, reveals homology with polypeptides involved in RNA processing.  |  Van Eynde, A., et al. 1995. J Biol Chem. 270: 28068-74. PMID: 7499293
8. Serine/threonine protein phosphatases.  |  Wera, S. and Hemmings, BA. 1995. Biochem J. 311 (Pt 1): 17-29. PMID: 7575450
9. Important roles for novel protein phosphatases dephosphorylating serine and threonine residues.  |  Cohen, PT. 1993. Biochem Soc Trans. 21: 884-8. PMID: 8132087
10. A regulatory subunit of smooth muscle myosin bound phosphatase.  |  Okubo, S., et al. 1994. Biochem Biophys Res Commun. 200: 429-34. PMID: 8166716
11. Structure and expression of a 72-kDa regulatory subunit of protein phosphatase 2A. Evidence for different size forms produced by alternative splicing.  |  Hendrix, P., et al. 1993. J Biol Chem. 268: 15267-76. PMID: 8392071
12. Protein serine/threonine phosphatases: structure, regulation, and functions in cell growth.  |  Mumby, MC. and Walter, G. 1993. Physiol Rev. 73: 673-99. PMID: 8415923

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PP2A-B56-γ Antibody (E-6) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-PP2A-B56-γ Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PP2A-B56-γ Antibody (E-6) Specifications

Alternate Product Name: PP2A phosphatase regulatory subunit B56 gamma

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 431-470

Epitope Target: PP2A-B56-γ

Epitope Species: human

Product Reactivity/Detection: PP2A-B56-γ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for PP2A-B56-γ Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin