PP2A-B56-β Antibody (E-6): sc-515676

PP2A-B56-β Antibody (E-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects PP2A-B56-β protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PP2A-B56-β (E-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PP2A-B56-β protein plays a crucial role in cellular processes through involvement in the protein phosphatase 2A (PP2A) complex, which dephosphorylates serine and threonine residues on target proteins. This dephosphorylation maintains cellular homeostasis, regulates cell division, and controls apoptosis, making PP2A-B56-β an essential player in various signaling pathways. PP2A-B56-β includes a regulatory subunit that interacts with the catalytic subunit of PP2A, determining substrate specificity and overall phosphatase complex activity. Understanding PP2A-B56-β structural dynamics provides insights into regulatory mechanisms and potential implications in diseases with disrupted phosphatase activity.

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PP2A-B56-β Antibody (E-6) References:

1. Structure and expression of two isoforms of the murine calmodulin-dependent protein phosphatase regulatory subunit (calcineurin B).  |  Ueki, K., et al. 1992. Biochem Biophys Res Commun. 187: 537-43. PMID: 1325794
2. The glycine 90 to aspartate alteration in the Abeta subunit of PP2A (PPP2R1B) associates with breast cancer and causes a deficit in protein function.  |  Esplin, ED., et al. 2006. Genes Chromosomes Cancer. 45: 182-90. PMID: 16276521
3. Negative regulation of Pim-1 protein kinase levels by the B56beta subunit of PP2A.  |  Ma, J., et al. 2007. Oncogene. 26: 5145-53. PMID: 17297438
4. Nuclear export and centrosome targeting of the protein phosphatase 2A subunit B56alpha: role of B56alpha in nuclear export of the catalytic subunit.  |  Flegg, CP., et al. 2010. J Biol Chem. 285: 18144-54. PMID: 20378546
5. Clk2 and B56β mediate insulin-regulated assembly of the PP2A phosphatase holoenzyme complex on Akt.  |  Rodgers, JT., et al. 2011. Mol Cell. 41: 471-9. PMID: 21329884
6. Protein phosphatase 2A regulatory subunit B56β modulates erythroid differentiation.  |  Wu, J., et al. 2016. Biochem Biophys Res Commun. 478: 1179-84. PMID: 27544028
7. Molecular cloning of NIPP-1, a nuclear inhibitor of protein phosphatase-1, reveals homology with polypeptides involved in RNA processing.  |  Van Eynde, A., et al. 1995. J Biol Chem. 270: 28068-74. PMID: 7499293
8. Serine/threonine protein phosphatases.  |  Wera, S. and Hemmings, BA. 1995. Biochem J. 311 (Pt 1): 17-29. PMID: 7575450
9. Important roles for novel protein phosphatases dephosphorylating serine and threonine residues.  |  Cohen, PT. 1993. Biochem Soc Trans. 21: 884-8. PMID: 8132087
10. A regulatory subunit of smooth muscle myosin bound phosphatase.  |  Okubo, S., et al. 1994. Biochem Biophys Res Commun. 200: 429-34. PMID: 8166716
11. Structure and expression of a 72-kDa regulatory subunit of protein phosphatase 2A. Evidence for different size forms produced by alternative splicing.  |  Hendrix, P., et al. 1993. J Biol Chem. 268: 15267-76. PMID: 8392071
12. Protein serine/threonine phosphatases: structure, regulation, and functions in cell growth.  |  Mumby, MC. and Walter, G. 1993. Physiol Rev. 73: 673-99. PMID: 8415923

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PP2A-B56-β Antibody (E-6) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-PP2A-B56-β Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PP2A-B56-β Antibody (E-6) Specifications

Alternate Product Name: PP2A phosphatase regulatory subunit B56 beta

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 449-472

Epitope Target: PP2A-B56-β

Epitope Species: human

Product Reactivity/Detection: PP2A-B56-β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for PP2A-B56-β Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin