PP2A-B56-β Antibody (E-6) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
specific for an epitope mapping between amino acids 449-472 near the C-terminus of PP2A-B56-β of human origin
Anti-PP2A-B56-beta Antibody (E-6) is recommended for detection of PP2A-B56-β of mouse, rat and human origin by WB, IP, IF and ELISA
Anti-PP2A-B56-beta Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PP2A-B56-β (E-6): sc-515676.
m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for PP2A-B56-β Antibody (E-6) for WB applications. This reagent is now offered in a bundle with PP2A-B56-β Antibody (E-6) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit, and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family. The PP2A family comprises subfamily members PP2Aα and PP2Aβ. The PP2A catalytic subunit associates with a variety of regulatory subunits. Regulatory subunits include PP2A-A-α and -A-β, PP2A-B-α and -B-β, PP2A-C-α and -C-β, PP2A-B56-α, -B56-β, -B56-γ and -B56-δ.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What blocking solution should I use with PP2A-B56-β (E-6): sc-515676 monoclonal antibody for Western blot?
Asked by: Professor Griffin
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here:
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