PP2A-B56-β Antibody (D-10): sc-515681

PP2A-B56-β Antibody (D-10) is a mouse monoclonal IgM antibody that detects PP2A-B56-β in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PP2A-B56-β is a crucial regulatory subunit of the protein phosphatase 2A (PP2A) complex, which plays a significant role in cellular signaling pathways by dephosphorylating serine and threonine residues on target proteins. This dephosphorylation is vital for regulating numerous cellular functions such as cell division, apoptosis, and maintaining cellular homeostasis. PP2A-B56-β specifically contributes to the assembly and substrate specificity of the PP2A holoenzyme, allowing interaction with a diverse array of proteins involved in critical cellular processes. Understanding PP2A-B56-β interactions with regulatory and catalytic partners is essential for elucidating roles in various signaling pathways and implications in diseases, including cancer and neurodegenerative disorders. Anti-PP2A-B56-β antibody (D-10) versatility in multiple applications makes this reagent an invaluable tool for researchers investigating complex regulatory mechanisms of protein phosphorylation and functional dynamics of the PP2A complex.

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PP2A-B56-β Antibody (D-10) References:

1. Protein phosphatase 2A and phosphatidylinositol 3-kinase regulate the activity of Sp1-responsive promoters.  |  Garcia, A., et al. 2000. J Biol Chem. 275: 9385-9. PMID: 10734082
2. Inhibition of B56-containing protein phosphatase 2As by the early response gene IEX-1 leads to control of Akt activity.  |  Rocher, G., et al. 2007. J Biol Chem. 282: 5468-77. PMID: 17200115
3. B56beta, a regulatory subunit of protein phosphatase 2A, interacts with CALEB/NGC and inhibits CALEB/NGC-mediated dendritic branching.  |  Brandt, N., et al. 2008. FASEB J. 22: 2521-33. PMID: 18385213
4. InAKTivation of insulin/IGF-1 signaling by dephosphorylation.  |  Narasimhan, SD., et al. 2009. Cell Cycle. 8: 3878-84. PMID: 19901535
5. Nuclear export and centrosome targeting of the protein phosphatase 2A subunit B56alpha: role of B56alpha in nuclear export of the catalytic subunit.  |  Flegg, CP., et al. 2010. J Biol Chem. 285: 18144-54. PMID: 20378546
6. Growth arrest of PPP2R5C and PPP2R5D double knockout mice indicates a genetic interaction and conserved function for these PP2A B subunits.  |  Dyson, JJ., et al. 2022. FASEB Bioadv. 4: 273-282. PMID: 35415460
7. Identification of a new family of protein phosphatase 2A regulatory subunits.  |  McCright, B. and Virshup, DM. 1995. J Biol Chem. 270: 26123-8. PMID: 7592815
8. The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm.  |  McCright, B., et al. 1996. J Biol Chem. 271: 22081-9. PMID: 8703017
9. Assignment of human protein phosphatase 2A regulatory subunit genes b56alpha, b56beta, b56gamma, b56delta, and b56epsilon (PPP2R5A-PPP2R5E), highly expressed in muscle and brain, to chromosome regions 1q41, 11q12, 3p21, 6p21.1, and 7p11.2 --> p12.  |  McCright, B., et al. 1996. Genomics. 36: 168-70. PMID: 8812429
10. Mapping of the gene encoding the B56 beta subunit of protein phosphatase 2A (PPP2R5B) to a 0.5-Mb region of chromosome 11q13 and its exclusion as a candidate gene for multiple endocrine neoplasia type 1 (MEN1).  |  Forbes, SA., et al. 1997. Hum Genet. 100: 481-5. PMID: 9272177

The preferred PP2A-B56-β antibody is PP2A-B56-β Antibody (E-6): sc-515676.