PP2A-B56-α Antibody (F-10): sc-271151

PP2A-B56-α Antibody (F-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects PP2A-B56-alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-PP2A-B56-α antibody (F-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PP2A-B56-alpha plays a crucial role in regulating cellular processes through involvement in serine and threonine residue dephosphorylation, vital for maintaining cellular homeostasis, regulating cell division, and controlling apoptosis. As a member of the protein phosphatase 2A (PP2A) family, PP2A-B56-alpha functions as a regulatory subunit interacting with the PP2A holoenzyme catalytic subunit, forming a trimeric complex essential for enzymatic activity. This interaction enables precise regulation of various signaling pathways, influencing numerous physiological processes and highlighting PP2A-B56-alpha′s significance in cellular function and potential implications in disease-related pathway disruptions.

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PP2A-B56-α Antibody (F-10) References:

1. Structure and expression of two isoforms of the murine calmodulin-dependent protein phosphatase regulatory subunit (calcineurin B).  |  Ueki, K., et al. 1992. Biochem Biophys Res Commun. 187: 537-43. PMID: 1325794
2. Protein phosphatase 2A regulatory subunit B56alpha associates with c-myc and negatively regulates c-myc accumulation.  |  Arnold, HK. and Sears, RC. 2006. Mol Cell Biol. 26: 2832-44. PMID: 16537924
3. Protein phosphatase 2A regulatory subunit B56α limits phosphatase activity in the heart.  |  Little, SC., et al. 2015. Sci Signal. 8: ra72. PMID: 26198358
4. Role of type 2A phosphatase regulatory subunit B56α in regulating cardiac responses to β-adrenergic stimulation in vivo.  |  Puhl, SL., et al. 2019. Cardiovasc Res. 115: 519-529. PMID: 30203051
5. Activation of the PP2A-B56α heterocomplex synergizes with venetoclax therapies in AML through BCL2 and MCL1 modulation.  |  Peris, I., et al. 2023. Blood. 141: 1047-1059. PMID: 36455198
6. Structural mechanism for inhibition of PP2A-B56α and oncogenicity by CIP2A.  |  Pavic, K., et al. 2023. Nat Commun. 14: 1143. PMID: 36854761
7. Molecular cloning of NIPP-1, a nuclear inhibitor of protein phosphatase-1, reveals homology with polypeptides involved in RNA processing.  |  Van Eynde, A., et al. 1995. J Biol Chem. 270: 28068-74. PMID: 7499293
8. Serine/threonine protein phosphatases.  |  Wera, S. and Hemmings, BA. 1995. Biochem J. 311 (Pt 1): 17-29. PMID: 7575450
9. Important roles for novel protein phosphatases dephosphorylating serine and threonine residues.  |  Cohen, PT. 1993. Biochem Soc Trans. 21: 884-8. PMID: 8132087
10. A regulatory subunit of smooth muscle myosin bound phosphatase.  |  Okubo, S., et al. 1994. Biochem Biophys Res Commun. 200: 429-34. PMID: 8166716
11. Structure and expression of a 72-kDa regulatory subunit of protein phosphatase 2A. Evidence for different size forms produced by alternative splicing.  |  Hendrix, P., et al. 1993. J Biol Chem. 268: 15267-76. PMID: 8392071
12. Protein serine/threonine phosphatases: structure, regulation, and functions in cell growth.  |  Mumby, MC. and Walter, G. 1993. Physiol Rev. 73: 673-99. PMID: 8415923

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PP2A-B56-α Antibody (F-10) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-PP2A-B56-α Antibody (F-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PP2A-B56-α Antibody (F-10) Specifications

Alternate Product Name: PP2A phosphatase regulatory subunit B56 alpha

Product Clone Name: F-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 459-486

Epitope Target: PP2A-B56-α

Epitope Species: human

Product Reactivity/Detection: PP2A-B56-α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for PP2A-B56-α Antibody (F-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin