PP2A-B55 Antibody (D-10): sc-365282

PP2A-B55 Antibody (D-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects PP2A-B55 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PP2A-B55 monoclonal antibody (D-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PP2A-B55 plays a crucial role in cellular signaling by regulating phosphorylation state of various substrates, which is essential for maintaining cellular homeostasis and controlling processes such as cell division and apoptosis. PP2A-B55 is part of the protein phosphatase 2A (PP2A) family, a major serine/threonine phosphatase involved in dephosphorylating proteins, thereby counteracting kinase action. B55 regulatory subunit family, which includes four isoforms (α, β, γ, and δ), influences substrate specificity and catalytic activity of PP2A complex, making PP2A-B55 vital for precise regulation of signaling pathways. PP2A-B55 interaction with various proteins, including kinases and other phosphatases, underscores PP2A-B55′s importance in cellular signaling networks and highlights potential as a therapeutic target in diseases characterized by dysregulated phosphorylation, such as cancer.

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PP2A-B55 Antibody (D-10) References:

1. Cloning and characterization of B delta, a novel regulatory subunit of protein phosphatase 2A.  |  Strack, S., et al. 1999. FEBS Lett. 460: 462-6. PMID: 10556517
2. Structure and expression of two isoforms of the murine calmodulin-dependent protein phosphatase regulatory subunit (calcineurin B).  |  Ueki, K., et al. 1992. Biochem Biophys Res Commun. 187: 537-43. PMID: 1325794
3. Structural, enzymatic and spatiotemporal regulation of PP2A-B55 phosphatase in the control of mitosis.  |  Lacroix, B., et al. 2022. Front Cell Dev Biol. 10: 967909. PMID: 36105360
4. PP2A-B55 phosphatase counteracts Ki-67-dependent chromosome individualization during mitosis.  |  Sanz-Flores, M., et al. 2024. Cell Rep. 43: 114494. PMID: 39003739
5. Substrate recognition principles for the PP2A-B55 protein phosphatase.  |  Kruse, T., et al. 2024. Sci Adv. 10: eadp5491. PMID: 39356758
6. Molecular cloning of NIPP-1, a nuclear inhibitor of protein phosphatase-1, reveals homology with polypeptides involved in RNA processing.  |  Van Eynde, A., et al. 1995. J Biol Chem. 270: 28068-74. PMID: 7499293
7. Serine/threonine protein phosphatases.  |  Wera, S. and Hemmings, BA. 1995. Biochem J. 311 (Pt 1): 17-29. PMID: 7575450
8. Important roles for novel protein phosphatases dephosphorylating serine and threonine residues.  |  Cohen, PT. 1993. Biochem Soc Trans. 21: 884-8. PMID: 8132087
9. A regulatory subunit of smooth muscle myosin bound phosphatase.  |  Okubo, S., et al. 1994. Biochem Biophys Res Commun. 200: 429-34. PMID: 8166716
10. Structure and expression of a 72-kDa regulatory subunit of protein phosphatase 2A. Evidence for different size forms produced by alternative splicing.  |  Hendrix, P., et al. 1993. J Biol Chem. 268: 15267-76. PMID: 8392071
11. Protein serine/threonine phosphatases: structure, regulation, and functions in cell growth.  |  Mumby, MC. and Walter, G. 1993. Physiol Rev. 73: 673-99. PMID: 8415923
12. Brain protein phosphatase 2A: developmental regulation and distinct cellular and subcellular localization by B subunits.  |  Strack, S., et al. 1998. J Comp Neurol. 392: 515-27. PMID: 9514514

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PP2A-B55 Antibody (D-10) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-PP2A-B55 Antibody (D-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PP2A-B55 Antibody (D-10) Specifications

Alternate Product Name: PP2A phosphatase B55 pan reactive

Product Clone Name: D-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 148-447

Epitope Target: PP2A-B55-α

Epitope Species: human

Product Reactivity/Detection: PP2A-B55-α, -β, -γ and -δ isoforms

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for PP2A-B55 Antibody (D-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin