PP1γ Antibody (E-4): sc-515943

PP1γ Antibody (E-4) is a mouse monoclonal IgG2a kappa light chain antibody engineered to target PP1γ, also known as PPP1CC or Protein Phosphatase 1 Gamma, a vital catalytic subunit of the protein phosphatase 1 (PP1) family. Anti-PP1γ antibody (E-4) recognizes a precise epitope mapping between amino acids 298-323 at the C-terminus of human PP1γ, ensuring accurate detection across mouse, rat, and human species using applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PP1γ is integral to cellular function due to its highly conserved structure, which includes a catalytic domain that binds essential metal ions like manganese or iron, facilitating phosphatase activity. This structural configuration allows PP1γ to interact with regulatory subunits and targeting proteins, enabling dephosphorylation of specific serine and threonine residues on target proteins. Such dephosphorylation is crucial for regulating numerous cellular processes, including cell cycle progression, transcriptional regulation, and apoptosis, thereby maintaining cellular homeostasis and responding to various cellular signals. The precise structural features of PP1γ dictate enzymatic activity and ability to form complexes with different regulatory proteins, which is essential for diverse signaling pathways. Dysregulation of PP1γ structure and function has been linked to several diseases, including cancer and neurodegenerative disorders, underscoring importance as a therapeutic target. PP1γ monoclonal antibody (E-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for a wide range of experimental applications. Anti-PP1γ antibody (E-4) supports advanced research into the structural and functional dynamics of protein phosphatases, contributing to a deeper understanding of their roles in health and disease and aiding in the development of targeted therapeutic strategies.

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PP1γ Antibody (E-4) References:

1. Repo-Man recruits PP1 gamma to chromatin and is essential for cell viability.  |  Trinkle-Mulcahy, L., et al. 2006. J Cell Biol. 172: 679-92. PMID: 16492807
2. PP1gamma2 and PPP1R11 are parts of a multimeric complex in developing testicular germ cells in which their steady state levels are reciprocally related.  |  Cheng, L., et al. 2009. PLoS One. 4: e4861. PMID: 19300506
3. Serotonin(1A)-receptor-dependent signaling proteins in mouse hippocampus.  |  Li, L., et al. 2009. Neuropharmacology. 57: 556-66. PMID: 19607848
4. Repo-Man coordinates chromosomal reorganization with nuclear envelope reassembly during mitotic exit.  |  Vagnarelli, P., et al. 2011. Dev Cell. 21: 328-42. PMID: 21820363
5. Cohesin Removal along the Chromosome Arms during the First Meiotic Division Depends on a NEK1-PP1γ-WAPL Axis in the Mouse.  |  Brieño-Enríquez, MA., et al. 2016. Cell Rep. 17: 977-986. PMID: 27760328
6. Unveiling the molecular mechanisms and developmental consequences of mercury (Hg) toxicity in zebrafish embryo-larvae: A comprehensive approach.  |  Henriques, MC., et al. 2023. Neurotoxicol Teratol. 100: 107302. PMID: 37739188
7. Molecular cloning of NIPP-1, a nuclear inhibitor of protein phosphatase-1, reveals homology with polypeptides involved in RNA processing.  |  Van Eynde, A., et al. 1995. J Biol Chem. 270: 28068-74. PMID: 7499293
8. Serine/threonine protein phosphatases.  |  Wera, S. and Hemmings, BA. 1995. Biochem J. 311 (Pt 1): 17-29. PMID: 7575450
9. Important roles for novel protein phosphatases dephosphorylating serine and threonine residues.  |  Cohen, PT. 1993. Biochem Soc Trans. 21: 884-8. PMID: 8132087
10. A regulatory subunit of smooth muscle myosin bound phosphatase.  |  Okubo, S., et al. 1994. Biochem Biophys Res Commun. 200: 429-34. PMID: 8166716
11. Structure and expression of a 72-kDa regulatory subunit of protein phosphatase 2A. Evidence for different size forms produced by alternative splicing.  |  Hendrix, P., et al. 1993. J Biol Chem. 268: 15267-76. PMID: 8392071
12. Protein serine/threonine phosphatases: structure, regulation, and functions in cell growth.  |  Mumby, MC. and Walter, G. 1993. Physiol Rev. 73: 673-99. PMID: 8415923

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PP1γ Antibody (E-4) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-PP1γ Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PP1γ Antibody (E-4) Specifications

Alternate Product Name: protein phosphatase 1 gamma

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 298-323

Epitope Target: PP1γ

Epitope Species: human

Product Reactivity/Detection: PP1γ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for PP1γ Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin