Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PP1β CRISPR/Cas9 KO Plasmid (m): sc-422378
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PP1β CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PP1β genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PP1β Antibody (A-6): sc-365678
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PP1β CRISPR/Cas9 KO Plasmid (m) | sc-422378 | 20 µg | $397.00 |
Overview
Ppp1cb encodes protein phosphatase 1 beta (PP1β), a catalytic subunit of serine/threonine protein phosphatase 1 that counterbalances kinase signaling by dephosphorylating diverse substrates in a context-dependent manner. PP1β participates in regulation of cell-cycle progression, cytoskeletal dynamics, and metabolic control through holoenzyme assembly with targeting and inhibitory subunits that direct subcellular localization and substrate specificity. In mouse systems, PP1β-linked phosphoregulation is commonly studied in pathways governing chromatin organization, mitotic exit, and stress-responsive signaling. Dysregulated PP1 activity and altered PP1β-containing complexes are frequently investigated for their roles in aberrant proliferation, neurobiological processes, and cardiometabolic phenotypes as mechanistic correlates in disease models.
PP1β CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ppp1cb gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ppp1cb together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ppp1cb open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PP1β protein expression.
This CRISPR knockout system enables efficient generation of Ppp1cb-deficient cell models for investigation of PP1β signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ppp1cb exon(s) critical for PP1β function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ppp1cb genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PP1β CRISPR/Cas9 KO Plasmid (m) and PP1β CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ppp1cb locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PP1β HDR Plasmid (m) and PP1β HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ppp1cb homology arms to support homology-directed repair at defined Ppp1cb target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.