POSH Antibody (E-1): sc-390103
- POSH Antibody (E-1) is a mouse monoclonal IgG1 κ POSH antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 845-883 near the C-terminus of POSH of mouse origin
- POSH Antibody (E-1) is recommended for detection of POSH and POSH2 of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and avian
- Anti-POSH Antibody (E-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for POSH Antibody (E-1) for WB applications. This reagent is now offered in a bundle with POSH Antibody (E-1) (see ordering information below).
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POSH Antibody (E-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects POSH protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). POSH (E-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. POSH, which stands for "plenty of SH3s," plays a crucial role in cellular signaling by interacting with small GTPases such as Rho, Rac, and Cdc42, which are essential for actin cytoskeleton dynamics. These GTPases function as molecular switches, toggling between active GTP-bound and inactive GDP-bound states, and their activation leads to significant rearrangements of filamentous actin, resulting in the formation of actin stress fibers. POSH specifically binds to the GTP-bound form of Rac, facilitating the activation of downstream signaling pathways, including c-Jun N-terminal kinase (JNK) and nuclear factor kappa B (NFkB), which are pivotal in regulating cell survival and apoptosis. POSH′s ability to modulate these pathways underscores its importance in various cellular processes, including cell migration, proliferation, and apoptosis, making POSH a valuable target for research in cancer and other diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
POSH Antibody (E-1) References:
- The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors. | Ridley, AJ. and Hall, A. 1992. Cell. 70: 389-99. PMID: 1643657
- Rho GTPases and signaling networks. | Van Aelst, L. and D'Souza-Schorey, C. 1997. Genes Dev. 11: 2295-322. PMID: 9308960
- A new rac target POSH is an SH3-containing scaffold protein involved in the JNK and NF-kappaB signalling pathways. | Tapon, N., et al. 1998. EMBO J. 17: 1395-404. PMID: 9482736
- The Rho small G protein family-Rho GDI system as a temporal and spatial determinant for cytoskeletal control. | Sasaki, T. and Takai, Y. 1998. Biochem Biophys Res Commun. 245: 641-5. PMID: 9588168
- The p21Rac/Cdc42-activated kinases (PAKs). | Knaus, UG. and Bokoch, GM. 1998. Int J Biochem Cell Biol. 30: 857-62. PMID: 9744077
- From Src Homology domains to other signaling modules: proposal of the 'protein recognition code'. | Sudol, M. 1998. Oncogene. 17: 1469-74. PMID: 9779993
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
POSH Antibody (E-1) | sc-390103 | 200 µg/ml | $322.00 | |||
POSH Antibody (E-1): m-IgG Fc BP-HRP Bundle | sc-526122 | 200 µg Ab; 10 µg BP | $361.00 | |||
POSH Antibody (E-1) AC | sc-390103 AC | 500 µg/ml, 25% agarose | $424.00 | |||
POSH Antibody (E-1) HRP | sc-390103 HRP | 200 µg/ml | $322.00 | |||
POSH Antibody (E-1) FITC | sc-390103 FITC | 200 µg/ml | $336.00 | |||
POSH Antibody (E-1) PE | sc-390103 PE | 200 µg/ml | $349.00 | |||
POSH Antibody (E-1) Alexa Fluor® 488 | sc-390103 AF488 | 200 µg/ml | $364.00 | |||
POSH Antibody (E-1) Alexa Fluor® 546 | sc-390103 AF546 | 200 µg/ml | $364.00 | |||
POSH Antibody (E-1) Alexa Fluor® 594 | sc-390103 AF594 | 200 µg/ml | $364.00 | |||
POSH Antibody (E-1) Alexa Fluor® 647 | sc-390103 AF647 | 200 µg/ml | $364.00 | |||
POSH Antibody (E-1) Alexa Fluor® 680 | sc-390103 AF680 | 200 µg/ml | $364.00 | |||
POSH Antibody (E-1) Alexa Fluor® 790 | sc-390103 AF790 | 200 µg/ml | $364.00 | |||
POSH (E-1) Neutralizing Peptide | sc-390103 P | 100 µg/0.5 ml | $69.00 |