Porimin Antibody (G-2): sc-377295
- Porimin Antibody (G-2) is a mouse monoclonal IgG2b κ Porimin antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 81-113 within an internal region of Porimin of mouse origin
- Porimin Antibody (G-2) is recommended for detection of Porimin of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Porimin Antibody (G-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Porimin (G-2): sc-377295.
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Porimin Antibody (G-2) for WB applications. These reagents are now offered in bundles with Porimin Antibody (G-2) (see ordering information below).
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Porimin Antibody (G-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects Porimin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Porimin (G-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Porimin, a 189 amino acid keratinocyte-associated pro-oncosis cell surface receptor, plays a critical role in mediating oncotic cell death by inducing membrane injury through rapid pore formation on the plasma membrane. This process is particularly significant as Porimin is associated with various forms of cell death, including apoptosis and cytolysis, and is characterized by organelle swelling, vacuolization, and increased membrane permeability. Porimin (G-2) monoclonal antibody recognizes a member of the cell membrane-associated Mucin family, distinguished by numerous O-linked and seven N-linked glycosylation sites on the extracellular domain, which are essential for function and stability. Notably, Porimin is expressed in all tissues except the ovary, with high expression levels observed in colorectal adenocarcinoma and lung carcinoma, indicating potential role in cancer biology. Porimin-mediated cell death is often preceded by cell aggregation and formation of membrane blebs, and may also inhibit cell adhesion, highlighting multifaceted role in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Porimin Antibody (G-2) References:
- Serial analysis of gene expression in differentiated cultures of human epidermal keratinocytes. | Jansen, BJ., et al. 2001. J Invest Dermatol. 116: 12-22. PMID: 11168793
- Molecular cloning of Porimin, a novel cell surface receptor mediating oncotic cell death. | Ma, F., et al. 2001. Proc Natl Acad Sci U S A. 98: 9778-83. PMID: 11481458
- Identification of novel genes for secreted and membrane-anchored proteins in human keratinocytes. | Bonkobara, M., et al. 2003. Br J Dermatol. 148: 654-64. PMID: 12752121
- A cell surface receptor defined by a mAb mediates a unique type of cell death similar to oncosis. | Zhang, C., et al. 1998. Proc Natl Acad Sci U S A. 95: 6290-5. PMID: 9600958
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Porimin Antibody (G-2) | sc-377295 | 200 µg/ml | $316.00 | |||
Porimin Antibody (G-2): m-IgGκ BP-HRP Bundle | sc-523271 | 200 µg Ab, 40 µg BP | $354.00 | |||
Porimin Antibody (G-2): m-IgG2b BP-HRP Bundle | sc-549102 | 200 µg Ab; 10 µg BP | $354.00 | |||
Porimin Antibody (G-2) AC | sc-377295 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Porimin Antibody (G-2) HRP | sc-377295 HRP | 200 µg/ml | $316.00 | |||
Porimin Antibody (G-2) FITC | sc-377295 FITC | 200 µg/ml | $330.00 | |||
Porimin Antibody (G-2) PE | sc-377295 PE | 200 µg/ml | $343.00 | |||
Porimin Antibody (G-2) Alexa Fluor® 488 | sc-377295 AF488 | 200 µg/ml | $357.00 | |||
Porimin Antibody (G-2) Alexa Fluor® 546 | sc-377295 AF546 | 200 µg/ml | $357.00 | |||
Porimin Antibody (G-2) Alexa Fluor® 594 | sc-377295 AF594 | 200 µg/ml | $357.00 | |||
Porimin Antibody (G-2) Alexa Fluor® 647 | sc-377295 AF647 | 200 µg/ml | $357.00 | |||
Porimin Antibody (G-2) Alexa Fluor® 680 | sc-377295 AF680 | 200 µg/ml | $357.00 | |||
Porimin Antibody (G-2) Alexa Fluor® 790 | sc-377295 AF790 | 200 µg/ml | $357.00 | |||
Porimin (G-2) Neutralizing Peptide | sc-377295 P | 100 µg/0.5 ml | $68.00 |