Pontin 52 Antibody (H-4): sc-365339
- Pontin 52 Antibody (H-4) is a mouse monoclonal IgM κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 123-153 within an internal region of Pontin 52 of human origin
- recommended for detection of Pontin 52 of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- TransCruz reagent for ChIP application (sc-365339 X, 200 µg/0.1 ml)
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Pontin 52 Antibody (H-4). This work is in progress.
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Pontin 52 Antibody (H-4) is a mouse monoclonal IgM antibody that detects Pontin 52 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Pontin 52, also known as RUVBL1 or NMP 238, is a crucial nuclear matrix protein primarily localized in the nucleoplasm of cells, while notably absent from the nucleoli. Anti-Pontin 52 antibody (H-4) plays a significant role in studying various cellular processes, including transcriptional regulation, due to Pontin 52′s ATPase/helicase motif, which suggests involvement as a cofactor in transcriptional activation pathways. Pontin 52 interacts with Myc, a well-known oncogene, and Reptin 52 (RUVBL2), indicating essential function in mediating Myc′s oncogenic transformation. The interaction between Pontin 52 and Myc is contingent upon a specific Myc domain that is critical for oncogenic activity, highlighting Pontin 52′s importance in cancer biology. The gene encoding human Pontin 52 is located on chromosome 3q21, further emphasizing relevance in genetic studies and potential therapeutic targets. Pontin 52 (H-4) antibody serves as an invaluable tool for researchers investigating the intricate roles of Pontin 52 in cellular mechanisms and disease states.
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LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Pontin 52 Antibody (H-4) References:
- The ATP-dependent helicase RUVBL1/TIP49a associates with tubulin during mitosis. | Gartner, W., et al. 2003. Cell Motil Cytoskeleton. 56: 79-93. PMID: 14506706
- Oligomeric assembly and interactions within the human RuvB-like RuvBL1 and RuvBL2 complexes. | Niewiarowski, A., et al. 2010. Biochem J. 429: 113-25. PMID: 20412048
- Suppression of Type I Interferon Signaling by E1A via RuvBL1/Pontin. | Olanubi, O., et al. 2017. J Virol. 91: PMID: 28122980
- RUVBL1, a novel C-RAF-binding protein, activates the RAF/MEK/ERK pathway to promote lung cancer tumorigenesis. | Guo, H., et al. 2018. Biochem Biophys Res Commun. 498: 932-939. PMID: 29545175
- Targeted deletion of the AAA-ATPase Ruvbl1 in mice disrupts ciliary integrity and causes renal disease and hydrocephalus. | Dafinger, C., et al. 2018. Exp Mol Med. 50: 1-17. PMID: 29959317
- Liver haploinsufficiency of RuvBL1 causes hepatic insulin resistance and enhances hepatocellular carcinoma progression. | Mello, T., et al. 2020. Int J Cancer. 146: 3410-3422. PMID: 31721195
- EIF3D promotes resistance to 5-fluorouracil in colorectal cancer through upregulating RUVBL1. | Li, C., et al. 2023. J Clin Lab Anal. 37: e24825. PMID: 36592991
- RUVBL1-modulated chromatin remodeling alters the transcriptional activity of oncogenic CTNNB1 in uveal melanoma. | Zhang, C. and Wu, S. 2023. Cell Death Discov. 9: 132. PMID: 37076452
- GART Functions as a Novel Methyltransferase in the RUVBL1/β-Catenin Signaling Pathway to Promote Tumor Stemness in Colorectal Cancer. | Tang, C., et al. 2023. Adv Sci (Weinh). 10: e2301264. PMID: 37439412
- Combined CRISPRi and proteomics screening reveal a cohesin-CTCF-bound allele contributing to increased expression of RUVBL1 and prostate cancer progression. | Tian, Y., et al. 2023. Am J Hum Genet. 110: 1289-1303. PMID: 37541187
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pontin 52 Antibody (H-4) | sc-365339 | 200 µg/ml | $322.00 | |||
Pontin 52 (H-4) Neutralizing Peptide | sc-365339 P | 100 µg/0.5 ml | $69.00 | |||
Pontin 52 Antibody (H-4) X | sc-365339 X | 200 µg/0.1 ml | $322.00 |