POLDIP3 CRISPR/Cas9 KO Plasmid (h): sc-413603
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- POLDIP3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the POLDIP3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: POLDIP3 Antibody (G-2): sc-398931
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
POLDIP3 CRISPR/Cas9 KO Plasmid (h) | sc-413603 | 20 µg | $397.00 |
Overview
POLDIP3 (polymerase delta interacting protein 3), also known as SKAR, is an RNA-binding protein that couples mRNA splicing to translation and supports efficient synthesis of proteins required for cell growth. It functions downstream of mTOR signaling, where phosphorylation-dependent interactions help coordinate translation initiation and ribosome recruitment to spliced transcripts. POLDIP3 has also been linked to regulation of genome maintenance pathways through interactions with DNA polymerase complexes, integrating proliferative cues with replication-associated processes. Dysregulation of these RNA processing and growth-control networks is relevant to studies of malignant transformation, cellular stress responses, and proliferative disorders.
POLDIP3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the POLDIP3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the POLDIP3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the POLDIP3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish POLDIP3 protein expression.
This CRISPR knockout system enables efficient generation of POLDIP3-deficient cell models for investigation of POLDIP3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting POLDIP3 exon(s) critical for POLDIP3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple POLDIP3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by POLDIP3 CRISPR/Cas9 KO Plasmid (h) and POLDIP3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the POLDIP3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by POLDIP3 HDR Plasmid (h) and POLDIP3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by POLDIP3 homology arms to support homology-directed repair at defined POLDIP3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.