POLA2 Antibody (D-2): sc-398255
- POLA2 Antibody (D-2) is a mouse monoclonal IgG1 κ POLA2 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 121-420 mapping within an internal region of POLA2 of human origin
- POLA2 Antibody (D-2) is recommended for detection of POLA2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-POLA2 Antibody (D-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for POLA2 Antibody (D-2) for WB applications. These reagents are now offered in bundles with POLA2 Antibody (D-2) (see ordering information below).
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SEE ALSO...
POLA2 Antibody (D-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects POLA2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). POLA2 (D-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. POLA2 protein, also known as DNA polymerase subunit alpha B, is a crucial component of the DNA polymerase alpha enzyme complex, which consists of four subunits: the large and small subunits of DNA primase, along with DNA polymerase subunits A and B. This complex plays a vital role in DNA replication, particularly during the early stages when coupling the primase/polymerase complex to the replication machinery, ensuring accurate and efficient DNA synthesis. POLA2 is composed of 598 amino acids, and incorporation into the enzyme complex is facilitated by its 250 amino acid N-terminal domain. Nuclear localization of POLA2 is essential for function, and phosphorylation may occur during the G2/M phase of the cell cycle, a modification that could regulate activity and interactions with other proteins involved in DNA replication.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
POLA2 Antibody (D-2) References:
- E2F mediates induction of the Sp1-controlled promoter of the human DNA polymerase epsilon B-subunit gene POLE2. | Huang, D., et al. 2001. Nucleic Acids Res. 29: 2810-21. PMID: 11433027
- DNA primase acts as a molecular brake in DNA replication. | Lee, JB., et al. 2006. Nature. 439: 621-4. PMID: 16452983
- Role of single-stranded DNA in targeting REV1 to primer termini. | Masuda, Y. and Kamiya, K. 2006. J Biol Chem. 281: 24314-21. PMID: 16803901
- The human GINS complex binds to and specifically stimulates human DNA polymerase alpha-primase. | De Falco, M., et al. 2007. EMBO Rep. 8: 99-103. PMID: 17170760
- Genome-wide analysis of the core DNA replication machinery in the higher plants Arabidopsis and rice. | Shultz, RW., et al. 2007. Plant Physiol. 144: 1697-714. PMID: 17556508
- Essential and non-essential DNA replication genes in the model halophilic Archaeon, Halobacterium sp. NRC-1. | Berquist, BR., et al. 2007. BMC Genet. 8: 31. PMID: 17559652
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
POLA2 Antibody (D-2) | sc-398255 | 200 µg/ml | $322.00 | |||
POLA2 Antibody (D-2): m-IgG Fc BP-HRP Bundle | sc-527757 | 200 µg Ab; 10 µg BP | $361.00 | |||
POLA2 Antibody (D-2): m-IgG1 BP-HRP Bundle | sc-533130 | 200 µg Ab; 20 µg BP | $361.00 | |||
POLA2 Antibody (D-2) AC | sc-398255 AC | 500 µg/ml, 25% agarose | $424.00 | |||
POLA2 Antibody (D-2) HRP | sc-398255 HRP | 200 µg/ml | $322.00 | |||
POLA2 Antibody (D-2) FITC | sc-398255 FITC | 200 µg/ml | $336.00 | |||
POLA2 Antibody (D-2) PE | sc-398255 PE | 200 µg/ml | $349.00 | |||
POLA2 Antibody (D-2) Alexa Fluor® 488 | sc-398255 AF488 | 200 µg/ml | $364.00 | |||
POLA2 Antibody (D-2) Alexa Fluor® 546 | sc-398255 AF546 | 200 µg/ml | $364.00 | |||
POLA2 Antibody (D-2) Alexa Fluor® 594 | sc-398255 AF594 | 200 µg/ml | $364.00 | |||
POLA2 Antibody (D-2) Alexa Fluor® 647 | sc-398255 AF647 | 200 µg/ml | $364.00 | |||
POLA2 Antibody (D-2) Alexa Fluor® 680 | sc-398255 AF680 | 200 µg/ml | $364.00 | |||
POLA2 Antibody (D-2) Alexa Fluor® 790 | sc-398255 AF790 | 200 µg/ml | $364.00 |