podoplanin Antibody (8.1.1): sc-53533

Podoplanin Antibody (8.1.1) is a Syrian hamster monoclonal IgG antibody engineered to detect Podoplanin, also known as glycoprotein 38 (gp38), T1-alpha, Aggrus, or PA2.26 antigen, specifically in mouse samples using western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) techniques. Anti-Podoplanin antibody (8.1.1) is raised against a thymic epithelial cell line of mouse origin. Podoplanin is a type I transmembrane glycoprotein predominantly localized in stromal cells of peripheral lymphoid tissues and thymic epithelial cells, where Podoplanin plays a crucial role in maintaining the structural integrity and unique morphology of podocytes in the kidney. The precise localization of Podoplanin in the cell membrane is essential for regulating lymphatic endothelial cell function and facilitating critical cell-cell interactions. Podoplanin monoclonal antibody (8.1.1) enables detailed studies of lymphangiogenesis, immune cell migration, and podocyte biology in murine models. Additionally, anti-Podoplanin antibody (8.1.1) is available in both non-conjugated form and a variety of conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, providing versatility for diverse experimental applications. Podoplanin (8.1.1) antibody serves as an invaluable tool for researchers exploring the multifaceted roles of Podoplanin in cellular structure and function within the immune and renal systems.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

podoplanin Antibody (8.1.1) References:

1. Lymphatic microvessel density and lymphovascular invasion assessed by anti-podoplanin immunostaining in human breast cancer.  |  Schoppmann, SF., et al. 2001. Anticancer Res. 21: 2351-5. PMID: 11724292
2. T1alpha, a lung type I cell differentiation gene, is required for normal lung cell proliferation and alveolus formation at birth.  |  Ramirez, MI., et al. 2003. Dev Biol. 256: 61-72. PMID: 12654292
3. Characterization of an antigenic determinant preferentially expressed by type I epithelial cells in the murine thymus.  |  Farr, A., et al. 1992. J Histochem Cytochem. 40: 651-64. PMID: 1374092
4. Characterization and cloning of a novel glycoprotein expressed by stromal cells in T-dependent areas of peripheral lymphoid tissues.  |  Farr, AG., et al. 1992. J Exp Med. 176: 1477-82. PMID: 1402691
5. Generation and characterization of telomerase-transfected human lymphatic endothelial cells with an extended life span.  |  Nisato, RE., et al. 2004. Am J Pathol. 165: 11-24. PMID: 15215158
6. IL-3 induces expression of lymphatic markers Prox-1 and podoplanin in human endothelial cells.  |  Gröger, M., et al. 2004. J Immunol. 173: 7161-9. PMID: 15585837
7. A transmembrane chemokine, CXC chemokine ligand 16, expressed by lymph node fibroblastic reticular cells has the potential to regulate T cell migration and adhesion.  |  Hara, T., et al. 2006. Int Immunol. 18: 301-11. PMID: 16410312
8. E11/gp38 selective expression in osteocytes: regulation by mechanical strain and role in dendrite elongation.  |  Zhang, K., et al. 2006. Mol Cell Biol. 26: 4539-52. PMID: 16738320

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-podoplanin Antibody (8.1.1) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-podoplanin Antibody (8.1.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

podoplanin Antibody (8.1.1) Specifications

Alternate Product Name: podoplanin

Product Clone Name: 8.1.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG

Amino Acids:

Epitope Target: podoplanin

Epitope Species: mouse

Product Reactivity/Detection: podoplanin

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for podoplanin Antibody (8.1.1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred podoplanin antibody is podoplanin Antibody (18H5): sc-59347.