PNPO Antibody (E-8): sc-393561
- PNPO Antibody (E-8) is a mouse monoclonal IgG1 κ PNPO antibody provided at 200 µg/ml
- raised against amino acids 157-261 mapping at the C-terminus of PNPO of human origin
- PNPO Antibody (E-8) is recommended for detection of PNPO of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PNPO Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PNPO Antibody (E-8) for WB applications. These reagents are now offered in bundles with PNPO Antibody (E-8) (see ordering information below).
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PNPO Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects PNPO protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-PNPO antibody (E-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PNPO, also known as pyridoxamine 5′-phosphate oxidase, is a crucial enzyme composed of 261 amino acids that plays a vital role in vitamin B6 metabolism. Specifically, PNPO catalyzes the conversion of pyridoxine 5′-phosphate (PNP) and pyridoxamine 5′-phosphate (PMP) into pyridoxal 5′-phosphate (PLP), the active form of vitamin B6, which is essential for numerous enzymatic reactions in amino acid metabolism and neurotransmitter synthesis. Proper functioning of PNPO is critical, as deficiencies can lead to serious neurological disorders, including PNPO-related neonatal epileptic encephalopathy and potential links to schizophrenia. Encoded by a gene located on human chromosome 17q21.32, PNPO is characterized by a structure that includes seven exons and six introns, with all exon/intron junctions adhering to the GT/AG consensus splicing site. PNPO′s regulatory region features Sp1-binding sites and CpG islands, indicative of its role as a housekeeping gene, while PNPO expression is developmentally regulated in tissues such as the liver, brain, skeletal muscle, and kidney, with minimal expression in the lung.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PNPO Antibody (E-8) References:
- Clinical and laboratory findings in twins with neonatal epileptic encephalopathy mimicking aromatic L-amino acid decarboxylase deficiency. | Bräutigam, C., et al. 2002. Neuropediatrics. 33: 113-7. PMID: 12200739
- Structure and properties of recombinant human pyridoxine 5'-phosphate oxidase. | Musayev, FN., et al. 2003. Protein Sci. 12: 1455-63. PMID: 12824491
- Genomic organization, tissue distribution and deletion mutation of human pyridoxine 5'-phosphate oxidase. | Kang, JH., et al. 2004. Eur J Biochem. 271: 2452-61. PMID: 15182361
- Neonatal epileptic encephalopathy caused by mutations in the PNPO gene encoding pyridox(am)ine 5'-phosphate oxidase. | Mills, PB., et al. 2005. Hum Mol Genet. 14: 1077-86. PMID: 15772097
- Pyridoxal 5'-phosphate may be curative in early-onset epileptic encephalopathy. | Hoffmann, GF., et al. 2007. J Inherit Metab Dis. 30: 96-9. PMID: 17216302
- Association between PNPO and schizophrenia in the Japanese population. | Song, H., et al. 2007. Schizophr Res. 97: 264-70. PMID: 17851041
- A new fatal case of pyridox(am)ine 5'-phosphate oxidase (PNPO) deficiency. | Ruiz, A., et al. 2008. Mol Genet Metab. 93: 216-8. PMID: 18024216
- Absence of pyridoxine-5'-phosphate oxidase (PNPO) activity in neoplastic cells: isolation, characterization, and expression of PNPO cDNA. | Ngo, EO., et al. 1998. Biochemistry. 37: 7741-8. PMID: 9601034
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PNPO Antibody (E-8) | sc-393561 | 200 µg/ml | $316.00 | |||
PNPO Antibody (E-8): m-IgG Fc BP-HRP Bundle | sc-530499 | 200 µg Ab; 10 µg BP | $354.00 | |||
PNPO Antibody (E-8): m-IgGκ BP-HRP Bundle | sc-524000 | 200 µg Ab, 40 µg BP | $354.00 | |||
PNPO Antibody (E-8): m-IgG1 BP-HRP Bundle | sc-544090 | 200 µg Ab; 20 µg BP | $354.00 | |||
PNPO Antibody (E-8) AC | sc-393561 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PNPO Antibody (E-8) HRP | sc-393561 HRP | 200 µg/ml | $316.00 | |||
PNPO Antibody (E-8) FITC | sc-393561 FITC | 200 µg/ml | $330.00 | |||
PNPO Antibody (E-8) PE | sc-393561 PE | 200 µg/ml | $343.00 | |||
PNPO Antibody (E-8) Alexa Fluor® 488 | sc-393561 AF488 | 200 µg/ml | $357.00 | |||
PNPO Antibody (E-8) Alexa Fluor® 546 | sc-393561 AF546 | 200 µg/ml | $357.00 | |||
PNPO Antibody (E-8) Alexa Fluor® 594 | sc-393561 AF594 | 200 µg/ml | $357.00 | |||
PNPO Antibody (E-8) Alexa Fluor® 647 | sc-393561 AF647 | 200 µg/ml | $357.00 | |||
PNPO Antibody (E-8) Alexa Fluor® 680 | sc-393561 AF680 | 200 µg/ml | $357.00 | |||
PNPO Antibody (E-8) Alexa Fluor® 790 | sc-393561 AF790 | 200 µg/ml | $357.00 |