PNP Antibody (H-7): sc-365081

PNP Antibody (H-7) is a mouse monoclonal IgG2b antibody that detects purine nucleoside phosphorylase (PNP) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PNP is a crucial enzyme that catalyzes the reversible phosphorolysis of ribonucleosides and 2′-deoxyribonucleosides, specifically targeting guanine and hypoxanthine, which are vital for nucleotide metabolism and cellular energy homeostasis. The enzyme forms a homotrimer and is part of the PNP/MTAP phosphorylase family, highlighting evolutionary significance. PNP′s proper functioning is essential, as deficiencies in this enzyme can lead to severe immunological and neurological disorders, particularly in children, where PNP deficiency is associated with a rare autosomal recessive genetic disease that compromises T-lymphocyte function. Such deficiency results in heightened susceptibility to infections, autoimmune diseases, and neurological impairments, making PNP research critical for understanding and potentially treating these serious health issues. Anti-PNP antibody (H-7) serves as an invaluable tool for researchers investigating PNP′s role in various biological processes and disease states.

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PNP Antibody (H-7) References:

1. Crystal structure of human PNP complexed with guanine.  |  de Azevedo, WF., et al. 2003. Biochem Biophys Res Commun. 312: 767-72. PMID: 14680831
2. PNP anticancer gene therapy.  |  Zhang, Y., et al. 2005. Curr Top Med Chem. 5: 1259-74. PMID: 16305530
3. Novel purine nucleoside analogues for T-cell-lineage acute lymphoblastic leukaemia and lymphoma.  |  Ravandi, F. and Gandhi, V. 2006. Expert Opin Investig Drugs. 15: 1601-13. PMID: 17107284
4. Tryptophan-free human PNP reveals catalytic site interactions.  |  Ghanem, M., et al. 2008. Biochemistry. 47: 3202-15. PMID: 18269249
5. Catalytic site conformations in human PNP by 19F-NMR and crystallography.  |  Suarez, J., et al. 2013. Chem Biol. 20: 212-22. PMID: 23438750
6. Preparation and evaluation of 99mTc-labeled porphyrin complexes prepared using PNP and HYNIC cores: studying the effects of core selection on pharmacokinetics and tumor uptake in a mouse model.  |  Guleria, M., et al. 2019. Medchemcomm. 10: 606-615. PMID: 31057740
7. PLGA-Based Nano-Encapsulation of Trachyspermum Ammi Seed Essential Oil (TSEO-PNP) as a Safe, Natural, Efficient, Anticancer Compound in Human HT-29 Colon Cancer Cell Line.  |  Almnhawy, M., et al. 2021. Nutr Cancer. 73: 2808-2820. PMID: 33319599
8. Structural characterization, antioxidant, and anti-inflammatory activity of polysaccharides from Plumula Nelumbinis.  |  Zheng, Q., et al. 2022. Int J Biol Macromol. 212: 111-122. PMID: 35594937
9. Myelin barrier breakdown, mechanical hypersensitivity, and painfulness in polyneuropathy with claudin-12 deficiency.  |  Chen, JT., et al. 2023. Neurobiol Dis. 185: 106246. PMID: 37527762
10. Human induced pluripotent stem cell/embryonic stem cell-derived pyramidal neuronal precursors show safety and efficacy in a rat spinal cord injury model.  |  Li, M., et al. 2024. Cell Mol Life Sci. 81: 318. PMID: 39073571

The preferred PNP antibody is PNP Antibody (F-10): sc-365551.