Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PNMTase CRISPR/Cas9 KO Plasmid (h): sc-406591
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PNMTase CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PNMTase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PNMTase Antibody (C-7): sc-393995
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PNMTase CRISPR/Cas9 KO Plasmid (h) | sc-406591 | 20 µg | $397.00 |
Overview
PNMT (phenylethanolamine N-methyltransferase) encodes PNMTase, the terminal enzyme in catecholamine biosynthesis that catalyzes conversion of norepinephrine to epinephrine using S-adenosyl-L-methionine as a methyl donor. PNMTase activity links adrenal medullary and sympathetic neuroendocrine signaling to cellular stress responses, integrating with glucocorticoid-regulated transcriptional programs and broader tyrosine metabolism. By modulating epinephrine availability, PNMT influences downstream adrenergic receptor signaling pathways that affect cardiovascular, metabolic, and neurobehavioral processes. Altered PNMT expression or regulation has been associated with dysregulated catecholamine homeostasis in stress-related phenotypes and endocrine/neuropsychiatric disease contexts.
PNMTase CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PNMT gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PNMT together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PNMT open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PNMTase protein expression.
This CRISPR knockout system enables efficient generation of PNMT-deficient cell models for investigation of PNMTase signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PNMT exon(s) critical for PNMTase function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PNMT genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PNMTase CRISPR/Cas9 KO Plasmid (h) and PNMTase CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PNMT locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PNMTase HDR Plasmid (h) and PNMTase HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PNMT homology arms to support homology-directed repair at defined PNMT target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.