PNMTase Antibody (C-7): sc-393995

PNMTase Antibody (C-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects PNMTase protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PNMTase antibody (C-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The phenylethanolamine N-methyltransferase (PNMT/PNMTase) enzyme plays a crucial role in the synthesis of epinephrine from norepinephrine, marking the final step in catecholamine biosynthesis, which is vital for the body′s response to stress and regulation of blood pressure. The human PNMT protein consists of 282 amino acids and exhibits significant homology with tyrosine hydroxylase, indicating a shared evolutionary pathway in catecholamine metabolism. PNMT expression is intricately regulated by hormonal and neural stimuli, as PNMT promoter contains sequences responsive to cholinergic and depolarization signals. Notably, cortisol and early growth response protein 1 (Egr-1) enhance PNMT expression, thereby influencing adrenaline production in pheochromocytomas, while protein kinase A up-regulates and protein kinase C down-regulates PNMT expression. Additionally, PNMT gene undergoes alternative splicing through a mechanism known as intron retention, resulting in two splice variants that are differentially expressed in a tissue-specific manner and regulated by glucocorticoids in the brain. The intron-less mRNA is predominantly expressed in the adrenal glands throughout development, while the intron-retained variant is constitutively expressed into adulthood. Importantly, variations in PNMT gene have been linked to an increased susceptibility to the sporadic form of early-onset Alzheimer′s disease, highlighting PNMT significance in both physiological and pathological contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PNMTase Antibody (C-7) References:

1. Tissue-specific alternative mRNA splicing of phenylethanolamine N-methyltransferase (PNMT) during development by intron retention.  |  Unsworth, BR., et al. 1999. Int J Dev Neurosci. 17: 45-55. PMID: 10219960
2. Differential involvement of PKA and PKC in regulation of catecholamine enzyme genes by PACAP.  |  Choi, HJ., et al. 1999. Peptides. 20: 817-22. PMID: 10477081
3. Enhanced expression of mRNA coding for the adrenaline-synthesizing enzyme phenylethanolamine-N-methyl transferase in adrenaline-secreting pheochromocytomas.  |  Isobe, K., et al. 2000. J Urol. 163: 357-62. PMID: 10604389
4. Neural regulation of phenylethanolamine N-methyltransferase (PNMT) gene expression in bovine chromaffin cells differs from other catecholamine enzyme genes.  |  Lee, YS., et al. 1999. J Mol Neurosci. 12: 53-68. PMID: 10636470
5. Phenylethanolamine N-methyltransferase (PNMT) gene and early-onset Alzheimer disease.  |  Mann, MB., et al. 2001. Am J Med Genet. 105: 312-6. PMID: 11378842
6. Therapeutic potential of Pnmt+ primer cells for neuro/myocardial regeneration.  |  Owji, A., et al. 2013. Am J Stem Cells. 2: 137-54. PMID: 24396707
7. Prenatal glucocorticoid exposure programs adrenal PNMT expression and adult hypertension.  |  Nguyen, P., et al. 2015. J Endocrinol. 227: 117-27. PMID: 26475702
8. Complete nucleotide and deduced amino acid sequence of bovine phenylethanolamine N-methyltransferase: partial amino acid homology with rat tyrosine hydroxylase.  |  Baetge, EE., et al. 1986. Proc Natl Acad Sci U S A. 83: 5454-8. PMID: 2874553
9. Novel cardiac cell subpopulations: Pnmt-derived cardiomyocytes.  |  Grassam-Rowe, A., et al. 2020. Open Biol. 10: 200095. PMID: 32810421
10. Molecular cloning of cDNA and chromosomal assignment of the gene for human phenylethanolamine N-methyltransferase, the enzyme for epinephrine biosynthesis.  |  Kaneda, N., et al. 1988. J Biol Chem. 263: 7672-7. PMID: 3372503
11. Intermittent Hypoxia Increased the Expression of DBH and PNMT in Neuroblastoma Cells via MicroRNA-375-Mediated Mechanism.  |  Takasawa, S., et al. 2022. Int J Mol Sci. 23: PMID: 35682548
12. The utility of the Poreh Nonverbal Memory Test (PNMT) for the detection of noncredible performance.  |  Braw, Y., et al. 2023. Appl Neuropsychol Adult. 1-8. PMID: 38086391

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PNMTase Antibody (C-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-PNMTase Antibody (C-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PNMTase Antibody (C-7) Specifications

Alternate Product Name: PNMTase

Product Clone Name: C-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 164-212

Epitope Target: PNMTase

Epitope Species: human

Product Reactivity/Detection: PNMTase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for PNMTase Antibody (C-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin